Adenovirus-mediated Wnt5a expression inhibits the telogen-to-anagen transition of hair follicles in mice.
Adenovirus-mediated Wnt5a expression inhibits the telogen-to-anagen transition of hair follicles in mice.
复制标题
腺病毒介导的 Wnt5a 表达抑制小鼠毛囊的休止期到毛发生长初期的转变
DOI：
10.7150/ijms.6137
复制
发表时间：
2013
影响因子：
3.6
通讯作者：
Yang T
中科院分区：
文献类型：
--
作者：
Xing YZ;Wang RM;Yang K;Guo HY;Deng F;Li YH;Ye JX;He L;Lian XH;Yang T
经典的Wnt/β-catenin通路在毛发周期诱导中起重要作用。Wnt 5a是一种非经典Wnt家族成员，通常拮抗其他系统中的经典Wnt信号传导。在毛囊中，Wnt 5a和典型Wnt都在休止期的细胞中表达。Wnt 5a已被证明对于控制毛细胞命运至关重要。然而，Wnt 5a在从休止期到生长期阶段的过渡中所起的作用是未知的。在这项研究中，使用整体安装原位杂交，我们表明，Wnt 5a是由其他几种细胞类型，毛乳头细胞除外，在整个头发周期。例如，Wnt 5a在休止期的隆突和次级毛生殖细胞中表达。我们的研究集中在脱毛的8周龄小鼠作为毛发生长的同步模型。有趣的是，腺病毒Wnt 5a在小鼠背部皮肤中的过表达导致休止期阶段的延长和生长期阶段的开始的抑制。然而，经过一段较长的时间，Wnt 5a诱导的无毛皮肤长出了四种毛皮毛发类型，这些新毛干的结构是正常的。利用基因芯片和定量芯片分析，我们发现Wnt 5a处理后β-catenin和经典Wnt信号转导的一些靶基因的表达降低。这些数据表明，Wnt 5a可以抑制休止期阶段，以维持毛囊的静止状态。
The canonical Wnt/β-catenin pathway plays an important role in hair cycle induction. Wnt5a is a non-canonical Wnt family member that generally antagonizes canonical Wnt signaling in other systems. In hair follicles, Wnt5a and canonical Wnt are both expressed in cells in the telogen stage. Wnt5a has been shown to be critical for controlling hair cell fate. However, the role that Wnt5a plays in the transition from the telogen to anagen stage is unknown. In this study, using whole-mount in situ hybridization, we show that Wnt5a is produced by several other cell types, excluding dermal papilla cells, throughout the hair cycle. For example, Wnt5a is expressed in bulge and secondary hair germ cells in the telogen stage. Our studies focused on the depilated 8-week-old mouse as a synchronized model of hair growth. Interestingly, overexpression of adenovirus Wnt5a in the dorsal skin of mice led to the elongation of the telogen stage and inhibition of the initiation of the anagen stage. However, following an extended period of time, four pelage hair types grew from hairless skin that was induced by Wnt5a, and the structure of these new hair shafts was normal. Using microarray analysis and quantitative arrays, we showed that the expression of β-catenin and some target genes of canonical Wnt signaling decreased after Wnt5a treatment. These data demonstrate that Wnt5a may inhibit the telogen stage to maintain a quiescent state of the hair follicle.
登录
查看更多内容
影响因子：
14.8
作者：
Luo, Jinyong;Deng, Zhong-Liang;He, Tong-Chuan
通讯作者：
He, Tong-Chuan
腺病毒介导的 Wnt10b 过表达诱导毛囊再生
影响因子：
6.5
作者：
Li, Yu-Hong;Zhang, Kun;Yang, Tian
通讯作者：
Yang, Tian
影响因子：
5.3
作者：
Dejmek, Janna;Safholm, Annette;Leandersson, Karin
通讯作者：
Leandersson, Karin
DOI：
10.1073/pnas.0704747104
发表时间：
2007-09-25
影响因子：
11.1
作者：
Nemeth, Michael J.;Topol, Lilia;Bodine, David M.
通讯作者：
Bodine, David M.
影响因子：
4
作者：
Alonso, L;Fuchs, E
通讯作者：
Fuchs, E