Electrochemical immunosensing on board microfluidic chip platforms
Electrochemical immunosensing on board microfluidic chip platforms
复制标题
DOI：
10.1016/j.trac.2011.06.020
复制
发表时间：
2012-01-01
影响因子：
13.1
通讯作者：
Escarpa, Alberto
中科院分区：
文献类型：
--
作者：
Hervas, Mirian;Lopez, Miguel A.;Escarpa, Alberto
近年来，作为免疫传感平台的微流控器件受到了极大的关注，成为生物医学、制药、环境和食品分析中的新兴技术。微流控平台的显着特点与免疫检测的结合产生了一个有前途的工具，选择性，灵敏，自动和即时检测在真实的application.In这篇文章中，我们回顾和讨论微流控材料基板，流体处理，复用，并主要是，自2005年以来，表面修饰和免疫试剂固定化策略的不同方面。虽然在这种微流控免疫传感平台中可以使用不同的检测模式，但电化学的特殊功能极大地扩展了这项技术，并且电化学检测在我们所综述的文章中是常见的。最后，我们对微流控免疫传感的未来前景进行了评论。(C)2011爱思唯尔有限公司保留所有权利。
Microfluidic devices as immunosensing platforms have had a great deal of attention in recent years, becoming an emergent technology in biomedical, pharmaceutical, environmental and food analysis. Combination of the remarkable features of microfluidic platforms with those of immunoassays produces a promising tool for selective, sensitive, automatic and point-of-care testing in real applications.In this article, we review and discuss different aspects of microfluidic material substrate, fluid handling, multiplexing, and, mainly, surface-modification and immunoreagent-immobilization strategies since 2005. Although different detection modes can be used in this kind of microfluidic immunosensing platform, the special features of electrochemistry have greatly expanded this technique, and electrochemical detection is common to the articles that we review. Finally, we comment on the future outlook for microfluidic immunosensing. (C) 2011 Elsevier Ltd. All rights reserved.