Biochemical diversity of glycosphingolipid biosynthesis as a driver of Coccolithovirus competitive ecology.
Biochemical diversity of glycosphingolipid biosynthesis as a driver of Coccolithovirus competitive ecology.
复制标题
鞘糖脂生物合成的生化多样性是球石病毒竞争生态的驱动力。
作者：
J. Nissimov;D. Talmy;L. Haramaty;Helen F. Fredricks;Ehud Zelzion;B. Knowles;A. M. Eren;Rebecca Vandzura;Christien P. Laber;B. Schieler;Christopher T Johns;K. More;M. Coolen;M. Follows;D. Bhattacharya;B. V. Van Mooy;K. Bidle
球石病毒 (EhV) 是一种含有双链 DNA 的大型病毒，可感染单细胞海洋球石藻赫胥氏石藻。考虑到赫胥黎大肠杆菌作为水华形成、钙化、光合自养生物的全球性和全球重要性，赫胥黎大肠杆菌与EhV相互作用在海洋碳生物地球化学中发挥着关键作用。病毒编码的鞘糖脂 (vGSL) 是由病毒编码的丝氨酸棕榈酰转移酶 (SPT) 活性产生的毒力因子。在这里，我们描述了一系列 EhV 菌株中 vGSL 的动态、多样性和催化产生与其 SPT 序列组成的关系，并探讨了它们是感染性和宿主死亡的决定因素的假设。在实验室实验中，vGSL 的产生和多样性与毒力、病毒复制率和裂解感染动态的增加呈正相关，但它们并不能解释毒力较低的 EhV 在自然 EhV 群落中的成功。与北大西洋受感染细胞相关的大多数 EhV 衍生的 SPT 扩增子序列源自感染速度较慢、毒性较低的 EhV。我们基于实验室、现场和数学模型的数据和模拟支持生态场景，即慢速感染、低毒力的 EhV 通过在活跃感染期间优先去除快速感染、毒力强的 EhV 或通过接触更广泛的宿主范围，成功地在北大西洋的赫胥氏埃里虫种群中竞争。
Coccolithoviruses (EhVs) are large, double-stranded DNA-containing viruses that infect the single-celled, marine coccolithophore Emiliania huxleyi. Given the cosmopolitan nature and global importance of E. huxleyi as a bloom-forming, calcifying, photoautotroph, E. huxleyi-EhV interactions play a key role in oceanic carbon biogeochemistry. Virally-encoded glycosphingolipids (vGSLs) are virulence factors that are produced by the activity of virus-encoded serine palmitoyltransferase (SPT). Here, we characterize the dynamics, diversity and catalytic production of vGSLs in an array of EhV strains in relation to their SPT sequence composition and explore the hypothesis that they are a determinant of infectivity and host demise. vGSL production and diversity was positively correlated with increased virulence, virus replication rate and lytic infection dynamics in laboratory experiments, but they do not explain the success of less-virulent EhVs in natural EhV communities. The majority of EhV-derived SPT amplicon sequences associated with infected cells in the North Atlantic derived from slower infecting, less virulent EhVs. Our lab-, field- and mathematical model-based data and simulations support ecological scenarios whereby slow-infecting, less-virulent EhVs successfully compete in North Atlantic populations of E. huxleyi, through either the preferential removal of fast-infecting, virulent EhVs during active infection or by having access to a broader host range.
带有 GAIP 的网格蛋白包被的囊泡在体外具有 GTP 酶激活蛋白活性。
DOI：
10.1073/pnas.96.12.6722
发表时间：
1999
影响因子：
11.1
作者：
Fischer,T;Elenko,E;McCaffery,JM;DeVries,L;Farquhar,MG
通讯作者：
Farquhar,MG
DOI：
10.1016/j.bbagen.2007.08.015
发表时间：
2008-03-01
影响因子：
3
作者：
Hakomori, Sen-Itiroh
通讯作者：
Hakomori, Sen-Itiroh