Structure and origin of a novel class of defective interfering particle of vesicular stomatitis virus.
Structure and origin of a novel class of defective interfering particle of vesicular stomatitis virus.
复制标题
一类新型水泡性口炎病毒缺陷干扰颗粒的结构和起源。
DOI：
10.1093/nar/12.6.2775
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发表时间：
1984
影响因子：
14.9
通讯作者：
Perrault,J
中科院分区：
文献类型：
--
作者：
Nichol,ST;O'Hara,PJ;Holland,JJ;Perrault,J
测定了水泡性口炎病毒复制回缺陷型干扰（DI）颗粒ST 1和一种新型复杂重排的速回（snapback）DI颗粒ST 2的基因组结构和末端序列。ST1DI基因组RNA具有54个碱基长的反向互补末端，其5′端与标准病毒基因组5′端同源。在该反向互补区域之后，DI RNA 5′端继续与标准病毒RNA 5′序列同源，而3′端分叉为病毒L基因内部序列内的序列。ST2 DI基因组RNA不含标准感染性病毒RNA 5′末端的共线性共价连接的正和负正义RNA拷贝，如从原型快速恢复DI结构预测的，而是似乎是ST1 DI RNA基因组的发夹拷贝。这是第一个证据表明DI颗粒可以从RNA模板而不是标准病毒RNA产生。世代模型和这些发现对RNA病毒进化的影响进行了讨论
The genome structure and terminal sequences of a ‘copyback’ defective interfering (DI) particle ST1, and a novel complexly rearranged ‘snapback’ DI particle ST2 of vesicular stomatitis virus have been determined. The ST1 DI genome RNA possesses 54 base long inverted complementary termini, the 5′ end of which is homologous to the standard virus genome 5′ end. Following this region of inverted complementarity the DI RNA 5′ end continues to be homologous to standard virus RNA 5′ sequences, whereas the 3′ end diverges into sequences within the virus L gene internal sequences. ST2 DI genome RNA does not contain colinear covalently linked plus and minus sense RNA copies of the standard infectious virus RNA 5′ terminus as predicted from the prototype snapback DI structure, but instead appears to be a hairpin copy of the ST1 DI RNA genome. This is the first evidence suggesting that DI particles may be generated from RNA templates other than the standard virus RNA. Generation models and the implications of these findings for RNA virus evolution are discussed