Global analysis of the yeast lipidome by quantitative shotgun mass spectrometry
Global analysis of the yeast lipidome by quantitative shotgun mass spectrometry
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DOI：
10.1073/pnas.0811700106
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发表时间：
2009-02-17
影响因子：
11.1
通讯作者：
Shevchenko, Andrej
中科院分区：
文献类型：
--
作者：
Ejsing, Christer S.;Sampaio, Julio L.;Shevchenko, Andrej
尽管一些真核模式生物的转录组、蛋白质组和相互作用组已经被详细描述，但脂质组仍然相对未被表征。以酿酒酵母为例，我们证明了自动散弹枪脂质组学分析能够通过流线型处理仅200万个酵母细胞的单个样本来实现脂质组范围内单个分子脂质的绝对定量。通过比较脂质组学，我们实现了覆盖21个主要脂类的250种分子脂质的绝对定量。该分析提供了接近95%的酵母脂质组覆盖率，与以前的方法相比，灵敏度提高了125倍。比较脂质组学表明，生长温度和脂质生物合成缺陷在酵母脂质组的整个分子组成中引起连锁反应。这项工作为真核脂质组的分子表征提供了资源，并建立了霰弹枪脂质组学作为补充生化研究和其他系统级方法的强大平台。
Although the transcriptome, proteome, and interactome of several eukaryotic model organisms have been described in detail, lipidomes remain relatively uncharacterized. Using Saccharomyces cerevisiae as an example, we demonstrate that automated shotgun lipidomics analysis enabled lipidome-wide absolute quantification of individual molecular lipid species by streamlined processing of a single sample of only 2 million yeast cells. By comparative lipidomics, we achieved the absolute quantification of 250 molecular lipid species covering 21 major lipid classes. This analysis provided approximate to 95% coverage of the yeast lipidome achieved with 125-fold improvement in sensitivity compared with previous approaches. Comparative lipidomics demonstrated that growth temperature and defects in lipid biosynthesis induce ripple effects throughout the molecular composition of the yeast lipidome. This work serves as a resource for molecular characterization of eukaryotic lipidomes, and establishes shotgun lipidomics as a powerful platform for complementing biochemical studies and other systems-level approaches.