High-resolution extracellular stimulation of dispersed hippocampal culture with high-density CMOS multielectrode array based on non-Faradaic electrodes
High-resolution extracellular stimulation of dispersed hippocampal culture with high-density CMOS multielectrode array based on non-Faradaic electrodes
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DOI：
10.1088/1741-2560/8/4/044003
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发表时间：
2011-08-01
影响因子：
4
通讯作者：
Shepard, K. L.
中科院分区：
文献类型：
--
作者：
Lei, N.;Ramakrishnan, S.;Shepard, K. L.
我们介绍了一种方法，电刺激单个神经元在任意时空模式的单细胞分辨率与精确控制刺激阈值。通过利用定制的微电子芯片，多达65000个非法拉第电极可以被唯一地寻址，电极密度超过6500个电极mm（-2）。我们展示了分散的原代海马神经元培养物的细胞外刺激，使用单细胞分辨率的芯片。
We introduce a method to electrically stimulate individual neurons at single-cell resolution in arbitrary spatiotemporal patterns with precise control over stimulation thresholds. By exploiting a custom microelectronic chip, up to 65 000 non-Faradaic electrodes can be uniquely addressed with electrode density exceeding 6500 electrodes mm(-2). We demonstrate extracellular stimulation of dispersed primary hippocampal neuronal cultures using the chip at single-cell resolution.