Metabolite profiling and quantification of phenolic compounds in methanol extracts of tomato fruit
Metabolite profiling and quantification of phenolic compounds in methanol extracts of tomato fruit
复制标题
DOI：
10.1016/j.phytochem.2010.08.002
复制
发表时间：
2010-11-01
期刊：
影响因子：
3.8
通讯作者：
Fernandez-Gutierrez, Alberto
中科院分区：
文献类型：
--
作者：
Gomez-Romero, Maria;Segura-Carretero, Antonio;Fernandez-Gutierrez, Alberto
西红柿和西红柿产品的消费与患某些类型癌症和其他慢性病的风险降低有关。这些有益的特性可能归因于关键代谢物的存在和它们之间的相互作用。我们开发并验证了一种分析方法，用于全面分析生长在西班牙东南部阿尔梅里亚的三个品种的生番茄(Daniela，Raf和Rambo)的甲醇提取物中的半极性代谢物。使用二极管阵列和飞行时间/离子陷阱质谱仪检测器来确保对具有高度不同性质的代谢物进行广泛检测。通过将其准确的质量信号与文献中报道的番茄化合物进行匹配，并通过UV和MS/MS信息、参考化合物和现有的代谢物数据库进行补充，从而对检测到的质量进行分配。通过这种方法，我们能够初步鉴定属于不同结构类别的135个化合物，其中21个化合物是我们所知的首次在番茄果实中报道的。在已鉴定的代谢物中，最丰富的是酚类化合物。由于其抗氧化活性和营养特性，这类次生代谢物引起了生产者和消费者的极大关注。它们的定量分析是通过使用每个家族密切相关的衍生品来实现的。(C)2010爱思唯尔有限公司。保留所有权利。
The consumption of tomatoes and tomato products has been associated with a reduction in the risk of contracting some types of cancer and other chronic diseases. These beneficial properties may be attributed to the presence of key metabolites and the interactions among them. We have developed and validated an analytical method for the comprehensive profiling of semi-polar metabolites in the methanol extract of three cultivars of raw tomatoes (Daniela, Raf and Rambo) grown in Almeria, in south-east Spain. Diode-array and time-of-flight/ion-trap mass spectrometry detectors were used to ensure the wide detection of metabolites with highly divergent properties. The masses thus detected were assigned by matching their accurate mass-signals with tomato compounds reported in the literature, and supplemented by UV and MS/MS information, reference compounds and existing metabolite databases. In this way we were able to identify tentatively 135 compounds belonging to various structural classes, 21 of which are to our knowledge reported for the first time in the tomato fruit. Among the metabolites identified, the most abundant were phenolic compounds. This class of secondary metabolites is attracting considerable attention from producers and consumers due to their antioxidant activity and nutritional properties. Their quantitative analysis was achieved by using closely related derivatives for each family. (C) 2010 Elsevier Ltd. All rights reserved.