Carotenoids affect proliferation of human prostate cancer cells
Carotenoids affect proliferation of human prostate cancer cells
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DOI：
10.1093/jn/131.12.3303
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发表时间：
2001-12-01
影响因子：
4.2
通讯作者：
Nagao, A
中科院分区：
文献类型：
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作者：
Kotake-Nara, E;Kushiro, M;Nagao, A
我们研究了食品中存在的各种类胡萝卜素是否可能参与人类前列腺癌的预防癌症作用。评估了 15 种类胡萝卜素对三种人类前列腺癌细胞系 PC-3、DU 145 和 LNCaP 活力的影响。当前列腺癌细胞在添加类胡萝卜素的培养基中以 20 μmol/L 培养 72 小时时，5,6-单环氧类胡萝卜素（即菠菜中的新黄质和褐藻中的岩藻黄质）使 PC-3 的细胞活力显着降低至 10.9% 和 14.9%，DU 145 的细胞活力显着降低至 10.9% 和 14.9%，DU 145 的细胞活力显着降低至 15.0% 和 5.0%，几乎为零和 5.0%。 LNCaP 分别为 9.8%。无环类胡萝卜素，如六氢番茄红素、zeta-胡萝卜素和番茄红素，都存在于番茄中，也会显着降低细胞活力。另一方面，八氢番茄红素、角黄素、β-隐黄素和玉米黄质并不影响前列腺癌细胞的生长。通过原位 TdT 介导的 dUTP 缺口末端标记 (TUNEL) 测定检测新黄质和岩藻黄质处理的细胞中细胞核的 DNA 片段化。新黄质和岩藻黄质被发现可通过诱导人前列腺癌细胞凋亡来降低细胞活力。这些结果表明，摄入富含新黄质和岩藻黄质的绿叶蔬菜和食用褐藻可能有降低前列腺癌风险的潜力。
We investigated whether various carotenoids present in foodstuffs were potentially involved in cancer-preventing action on human prostate cancer. The effects of 15 kinds of carotenoids on the viability of three lines of human prostate cancer cells, PC-3, DU 145 and LNCaP, were evaluated. When the prostate cancer cells were cultured in a carotenoid-supplemented medium for 72 h at 20 mu mol/L, 5,6-monoepoxy carotenoids, namely, neoxanthin from spinach and fucoxanthin from brown algae, significantly reduced cell viability to 10.9 and 14.9% for PC-3, 15.0 and 5.0% for DU 145, and nearly zero and 9.8% for LNCaP, respectively. Acyclic carotenoids such as phytofluene, zeta -carotene and lycopene, all of which are present in tomato, also significantly reduced cell viability. On the other hand, phytoene, canthaxanthin, beta -cryptoxanthin and zeaxanthin did not affect the growth of the prostate cancer cells. DNA fragmentation of nuclei in neoxanthin- and fucoxanthin-treated cells was detected by in situ TdT-mediated dUTP nick end labeling (TUNEL) assay. Neoxanthin and fucoxanthin were found to reduce cell viability through apoptosis induction in the human prostate cancer cells. These results suggest that ingestion of leafy green vegetables and edible brown algae rich in neoxanthin and fucoxanthin might have the potential to reduce the risk of prostate cancer.