Establishment of a Primary Pleomorphic Xanthoastrocytoma Cell Line: In Vitro Responsiveness to Some Chemotherapeutics
Establishment of a Primary Pleomorphic Xanthoastrocytoma Cell Line: In Vitro Responsiveness to Some Chemotherapeutics
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DOI：
10.1227/neu.0b013e3182262c5b
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发表时间：
2012-01-01
期刊：
影响因子：
4.8
通讯作者：
Ercan, Sevim
中科院分区：
文献类型：
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作者：
Bagriacik, Emin Umit;Baykaner, Mustafa Kemali;Ercan, Sevim
背景：间变性多形性黄色星形细胞瘤是一种生长迅速、恶性、最终致命的中枢神经系统肿瘤。在体外条件下检测化疗药物的敏感性将是一个有用的策略，以确定敏感或耐药药物的致命brain cancer.Objective：建立原代细胞培养的切除肿瘤组织从多形性黄色星形细胞瘤轴承和测试其对各种抗癌化疗药物的敏感性。将从患有复发性3级多形性黄色星形细胞瘤的患者切除的肿瘤组织制备的悬浮液在培养皿中培养，直到细胞开始生长。免疫荧光和免疫组织化学可视化进行共聚焦和光学显微镜。用MTT法（3-（4，5-dimethylthiazol-2-yl）-2，5-diphenyltetrazoliumbromide）和~ 3 H-胸腺嘧啶核苷掺入法（3 H-tymidine intruding）检测几种抗癌药物的细胞毒性。结论：手术切除肿瘤组织的原代细胞培养可用于复发性恶性脑肿瘤，特别是间变性多形性黄色星形细胞瘤的肿瘤生物学和化疗药物敏感性的研究。
BACKGROUND: Anaplastic pleomorphic xanthoastrocytoma is an aggressively growing, malignant, and eventually fatal tumor of the central nervous system. Testing chemotherapeutic drug sensitivity under in vitro conditions would be a useful strategy to determine sensitive or resistant drugs for fatal brain cancers.OBJECTIVE: To establish primary cell cultures of excised tumor tissue from pleomorphic xanthoastrocytoma-bearing patients and to test their sensitivity against various anticancer chemotherapy drugs.METHODS: Prepared suspensions of the excised tumor tissue from a patient who had a recurrent grade 3 pleomorphic xanthoastrocytoma was cultured in culture dishes until cells began to grow. Immunofluorescent and immunohistochemical visualizations were performed using confocal and light microscopy. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay in comparison with 3 H-thymidine incorporation assay was used to test cellular toxicity of several anticancer drugs.RESULTS: We established vigorously growing primary cells of the tumor. Drug sensitivity testing was conducted successfully.CONCLUSION: Primary cell cultures of surgically removed tumor tissues may be useful in studies of cancer biology and chemotherapeutic drug sensitivity for recurrent malignant brain tumors, particularly for anaplastic pleomorphic xanthoastrocytoma.