OVARY AS A SOURCE OF ALPHA-ECDYSONE IN AN ADULT MOSQUITO
OVARY AS A SOURCE OF ALPHA-ECDYSONE IN AN ADULT MOSQUITO
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DOI：
10.1073/pnas.72.8.3255
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发表时间：
1975-01-01
影响因子：
11.1
通讯作者：
BOHM, MK
中科院分区：
文献类型：
--
作者：
HAGEDORN, HH;OCONNOR, JD;BOHM, MK
在体外培养的埃及伊蚊的卵巢分泌的物质在放射免疫分析中表现为蜕皮激素。经高效液相和气-液色谱鉴定为α-蜕皮激素。通过生物测定和直接测定，血餐后16小时分泌物达到最大值。卵巢切除降低了成人血餐后蜕皮激素的浓度。全身提取物的定性分析表明，β-蜕皮激素是存在的主要物种。因此，卵巢似乎会分泌一种前激素--α-蜕皮激素，它会转化为β-蜕皮激素。β-蜕皮激素在刺激成年蚊子的卵子发育方面发挥着重要作用，并可能在其他昆虫中发挥生殖作用。
The ovaries of the mosquito Aedes aegypti cultured in vitro secrete material that behaves like ecdysone in a radioimmunoassay. The material was identified as alpha-ecdysone by high-resolution liquid and gas-liquid chromatography. Secretion reached a maximum 16 hr after a blood meal as shown by bioassay and direct determination. Ovariectomy reduced the concentration of ecdysone in the adult after a blood meal. Qualitative analysis of whole-body extracts indicated beta-ecdysone to be the principal species present. Thus the ovaries appear to secrete a prohormone, alpha-ecdysone, which is converted to beta-ecdysone. Beta-ecdysone plays a significant role in stimulating egg development in the adult mosquito and may have reproductive roles in other insects.