Smr: a bacterial and eukaryotic homologue of the C-terminal region of the MutS2 family
Smr: a bacterial and eukaryotic homologue of the C-terminal region of the MutS2 family
复制标题
DOI：
10.1016/s0968-0004(99)01419-x
复制
发表时间：
1999-08-01
影响因子：
13.8
通讯作者：
Philippe, H
中科院分区：
文献类型：
--
作者：
Moreira, D;Philippe, H
299种蛋白质。为了研究仅具有mutS 1家族基因的物种是否完全缺乏这种保守序列，我们使用B的90个残基C-末端搜索了非冗余NCBI蛋白质数据库。枯草杆菌MutS 2序列作为迭代PSI-Blast搜索的查询（http：//www 3. ncbi. nlm。nih。gov/cgi-bin/BLAST/nph-psi_blast）。有趣的是，在第一次迭代后产生显著比对的序列中，我们在大肠杆菌和梅毒螺旋体中鉴定出一个小的（~ 200个残基）蛋白，该蛋白含有该保守区域（E值分别为10 6和10 5）。当这两个序列与MutS 2序列一起被包括在用于第二次迭代的PSI-Blast查询中时，第二个E.大肠杆菌同源物的检索具有显著性评分（E10 5）。一种可能的同源物也被检测到，
299 proteins. To investigate whether species that possess only genes of the mutS1 family lack this conserved sequence completely, we searched the nonredundant NCBI protein database, using the 90 residue C-terminus of the B. subtilis MutS2 sequence as a query for an iterated PSI-Blast search (http://www3. ncbi. nlm. nih. gov/cgi-bin/BLAST/nph-psi_blast) with default parameters. Interestingly, among the sequences that produced significant alignments after the first iteration, we identified a small (~ 200 residue) protein in Escherichia coli and Treponema pallidum that contains this conserved region (E values were 10 6 and 10 5, respectively). When these two sequences are included in the PSI-Blast query for the second iteration, together with the MutS2 sequences, a second E. coli homologue is retrieved with a significant score (E 10 5). A likely homologue is also detected in