Complete mitochondrial genome sequences for Crown-of-thorns starfish Acanthaster planci and Acanthaster brevispinus
Complete mitochondrial genome sequences for Crown-of-thorns starfish Acanthaster planci and Acanthaster brevispinus
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DOI：
10.1186/1471-2164-7-17
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发表时间：
2006-01-27
期刊：
影响因子：
4.4
通讯作者：
Nadaoka, K
中科院分区：
文献类型：
--
作者：
Yasuda, N;Hamaguchi, M;Nadaoka, K
背景：棘冠海星（学名：Acroaster planci（L.））一直被认为是印度洋-太平洋地区大量珊瑚礁系统珊瑚死亡的原因。由于其高繁殖力和浮游幼虫阶段的持续时间长，爆发的机制可能与其元种群动态，这应该通过幼虫采样和种群遗传分析来检查。然而，A. planci幼虫与其它小行星幼虫相比，形态特征不明显，因此很难区分A.浮游生物样本中没有物种特异性标记的浮游生物幼虫。此外，没有工具可以揭示A的传播途径。浮游幼虫因此，高度多态性遗传标记的发展有可能克服这些困难。为了获得这些目的的基因组信息，对A. planci及其姊妹种A.结果：短棘棘天牛的完整线粒体DNA已被测定，并讨论了它们的特征。planci和A.短刺线虫和短刺线虫的长度分别为16，234 bp和16，254 bp。这些值属于其他后生动物线粒体基因组报告的长度变化范围。它们含有13种蛋白质，2种rRNA和22种tRNA基因，以及与小行星Asterina pectinifera相同顺序的推定控制区。A. planci和A.短刺A. brevispinus的L链上编码大部分蛋白质编码基因的氨基酸序列分别为56.3%和56.4%，低于短刺A.果胶类占61.2%。A. planci和A.在13个蛋白质编码基因中，短刺酵母的CO2和CO3区的编码率最高（均为90.6%），ND2基因的编码率最低（84.2%）。在推测的氨基酸序列中，CO1高度保守（99.2%），ATP 8的进化速度明显快于其它蛋白质编码基因（85.2%）。planci与A.短棘肌然而，A. planci和A.短棘肌线粒体DNA全序列的测定对于研究幼虫发生、幼虫检测和种群遗传学具有重要意义。
Background: The crown-of-thorns starfish, Acanthaster planci ( L.), has been blamed for coral mortality in a large number of coral reef systems situated in the Indo-Pacific region. Because of its high fecundity and the long duration of the pelagic larval stage, the mechanism of outbreaks may be related to its meta-population dynamics, which should be examined by larval sampling and population genetic analysis. However, A. planci larvae have undistinguished morphological features compared with other asteroid larvae, hence it has been difficult to discriminate A. planci larvae in plankton samples without species-specific markers. Also, no tools are available to reveal the dispersal pathway of A. planci larvae. Therefore the development of highly polymorphic genetic markers has the potential to overcome these difficulties. To obtain genomic information for these purposes, the complete nucleotide sequences of the mitochondrial genome of A. planci and its putative sibling species, A. brevispinus were determined and their characteristics discussed.Results: The complete mtDNA of A. planci and A. brevispinus are 16,234 bp and 16,254 bp in size, respectively. These values fall within the length variation range reported for other metazoan mitochondrial genomes. They contain 13 proteins, 2 rRNA, and 22 tRNA genes and the putative control region in the same order as the asteroid, Asterina pectinifera. The A + T contents of A. planci and A. brevispinus on their L strands that encode the majority of protein-coding genes are 56.3% and 56.4% respectively and are lower than that of A. pectinifera (61.2%). The percent similarity of nucleotide sequences between A. planci and A. brevispinus is found to be highest in the CO2 and CO3 regions ( both 90.6%) and lowest in ND2 gene (84.2%) among the 13 protein-coding genes. In the deduced putative amino acid sequences, CO1 is highly conserved (99.2%), and ATP8 apparently evolves faster any of the other protein-coding gene (85.2%).Conclusion: The gene arrangement, base composition, codon usage and tRNA structure of A. planci are similar to those of A. brevispinus. However, there are significant variations between A. planci and A. brevispinus. Complete mtDNA sequences are useful for the study of phylogeny, larval detection and population genetics.