The fusion of genomes leads to more options: A comparative investigation on the desulfo-glucosinolate sulfotransferases of Brassica napus and homologous proteins of Arabidopsis thaliana.
The fusion of genomes leads to more options: A comparative investigation on the desulfo-glucosinolate sulfotransferases of Brassica napus and homologous proteins of Arabidopsis thaliana.
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DOI：
10.1016/j.plaphy.2015.03.009
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发表时间：
2015-06
期刊：
影响因子：
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通讯作者：
F. Hirschmann;J. Papenbrock
中科院分区：
文献类型：
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作者：
F. Hirschmann;J. Papenbrock
磺基转移酶(SOT)(EC 2.8.2.-)通过催化核心芥子油苷形成的最后步骤，在芥子油苷(GI)生物合成中发挥关键作用。在拟南芥中，之前对三种脱硫（ds）-Gl SOT AtSOT16、AtSOT17 和 AtSOT18 进行了表征，显示出与 ds-Gls 不同的亲和力。但是关于这些 SOT 的知识是否可以普遍转移到其他 GL 合成工厂？研究了经济相关作物甘蓝型油菜中存在多少 SOT，以及是否可以通过序列分析预测其特征。最近的序列dB。 油菜（Brassica rapa）和甘蓝（Brassica oleracea）的杂交种。通过数据库研究，鉴定出 71 个假定的功能性 BnSOT 家族成员，其中至少 11 个是假定的 ds-G1 SOT。除了 AtSOT16 – 18 的同源物外，系统发育分析还揭示了 ds-G1 SOT 的新亚科，这些亚科在 A.AtSOT16-18 中不存在。 拟南芥。三个 B. 表达和纯化napusds-G1 SOT蛋白，并通过确定对不同ds-Gls的底物亲和力来表征。其中两个，BnSOT16-a 和 BnSOT16-b，与吲哚 ds-G1 表现出显着更高的亲和力，与 AtSOT16 类似。此外，与 AtSOT17 类似，BnSOT17-a 也被表征并显示出对长链脂肪族 Gls 更高的亲和力。 AtSOT18 同源物的鉴定不太可靠，因为假定的 SOT18 序列更加异质，并且不可能确认相似的特征。
Sulfotransferases (SOTs) (EC 2.8.2.-) play a crucial role in the glucosinolate (Gl) biosynthesis, by catalyzing the final step of the core glucosinolate formation. In Arabidopsis thaliana the three desulfo (ds)-Gl SOTs AtSOT16, AtSOT17 and AtSOT18 were previously characterized, showing different affinities to ds-Gls. But can the knowledge about these SOTs be generally transferred to other Gl-synthesizing plants? It was investigated how many SOTs are present in the economically relevant crop plant Brassica napus L., and if it is possible to predict their characteristics by sequence analysis. The recently sequencedB. napusis a hybrid of Brassica rapa and Brassica oleracea. By database research, 71 putative functional BnSOT family members were identified and at least eleven of those are putative ds-Gl SOTs. Besides the homologs of AtSOT16 – 18, phylogenetic analyses revealed new subfamilies of ds-Gl SOTs, which are not present inA. thaliana. Three of theB. napusds-Gl SOT proteins were expressed and purified, and characterized by determining the substrate affinities to different ds-Gls. Two of them, BnSOT16-a and BnSOT16-b, showed a significantly higher affinity to an indolic ds-Gl, similarly to AtSOT16. Additionally, BnSOT17-a was characterized and showed a higher affinity to long chained aliphatic Gls, similarly to AtSOT17. Identification of homologs to AtSOT18 was less reliable, because putative SOT18 sequences are more heterogeneous and confirmation of similar characteristics was not possible.