Conjugated linoleic acid production from castor oil by Lactobacillus plantarum JCM 1551
Conjugated linoleic acid production from castor oil by Lactobacillus plantarum JCM 1551
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DOI：
10.1016/j.enzmictec.2004.03.013
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发表时间：
2004-07-06
影响因子：
3.4
通讯作者：
Shimizu, S
中科院分区：
文献类型：
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作者：
Ando, A;Ogawa, J;Shimizu, S
以植物乳杆菌JCM 1551洗涤细胞为催化剂，研究了蓖麻油以蓖麻油酸为主要脂肪酸成分的共轭亚油酸（CLA）的生产条件。在脂肪酶存在下，蓖麻油成为细菌生产CLA的有效底物。脂肪酶M“天野”10对CLA产生的支持作用是最有效的。添加洗涤剂，特别是Lubrol PX，提高了CLA的产量。合成的CLA由顺式-9反式-11-十八二烯酸（CLAI）和反式-9反式-11-十八二烯酸（CLA2）两种异构体组成。在最佳条件（1.0 M柠檬酸钠缓冲液，pH 6.0, 37℃）下，以蓖麻油为底物，以洗过的L. plantarum细胞（12%，湿细胞w/v）为催化剂，5.0 mg/ml蓖麻油在99 h（产率0.027 mg/ml/h）可制得2.7 mg/ml CLA， 30 mg/ml蓖麻油在171 h（产率0.044 mg/ml/h）可制得7.5 mg/ml CLA。前者产CLA占总脂肪酸的45.5%，由cl1（26%）和cl2（74%）组成。(C) 2004爱思唯尔公司版权所有。
The conditions for conjugated linoleic acid (CLA) production from castor oil, in which the main fatty acid component is ricinoleic acid, were investigated using washed cells of Lactobacillus plantarum JCM 1551 as the catalyst. In the presence of lipase, castor oil became an effective substrate for CLA production by the bacterium. Lipase M "Amano" 10 supported CLA production most effectively among the lipases tested. The addition of a detergent, especially Lubrol PX, enhanced the CLA production. The CLA produced comprised a mixture of two isomers, i.e. cis-9,trans-11-octadecadienoic acid (CLAI) and trans-9,trans-11-octadecadienoic acid (CLA2). Under the optimum conditions (1.0 M sodium citrate buffer, pH 6.0, 37 degreesC) with castor oil as the substrate and washed cells of L. plantarum (12%, wet cell w/v) as the catalyst, 2.7 mg/ml CLA was produced from 5.0 mg/ml castor oil in 99 h (productivity, 0.027 mg/ml/h), and 7.5 mg/ml CLA from 30 mg/ml castor oil in 171 h (productivity, 0.044 mg/ml/h). In the former case, the CLA produced accounted for 45.5% of the total fatty acids obtained, and consisted of CLA1 (26%) and CLA2 (74%). (C) 2004 Elsevier Inc. All rights reserved.