The Arp2/3 complex branches filament barbed ends: functional antagonism with capping proteins
The Arp2/3 complex branches filament barbed ends: functional antagonism with capping proteins
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DOI：
10.1038/35017011
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发表时间：
2000-07-01
影响因子：
21.3
通讯作者：
Carlier, MF
中科院分区：
文献类型：
--
作者：
Pantaloni, D;Boujemaa, R;Carlier, MF
Arp2/3 复合物是响应信号传导的肌动蛋白聚合的重要调节因子，并在板状伪足中产生树突状丝状阵列。在这里，我们展示了激活的 Arp2/3 复合物与细丝的倒刺末端相互作用以启动倒刺末端分支。 Arp2/3 的倒刺末端支化定量地解释了聚合动力学以及通过电子显微镜观察到的细丝分支的长度相关性。在重组运动测定中，李斯特菌表面的细丝分支可见。 Arp2/3 复合物和加帽蛋白之间的功能拮抗作用对于维持肌动蛋白组装和基于肌动蛋白的运动的稳定状态至关重要。
The Arp2/3 complex is an essential regulator of actin polymerization in response to signalling and generates a dendritic array of filaments in lamellipodia. Here we show that the activated Arp2/3 complex interacts with the barbed ends of filaments to initiate barbed-end branching. Barbed-end branching by Arp2/3 quantitatively accounts for polymerization kinetics and for the length correlation of the branches of filaments observed by electron microscopy. Filament branching is Visualized at the surface of Listeria in a reconstituted motility assay. The functional antagonism between the Arp2/3 complex and capping proteins is essential in the maintenance of the steady state of actin assembly and actin-based motility.