Downregulation of runt‐related transcription factor 3 associated with poor prognosis of adenoid cystic and mucoepidermoid carcinomas of the salivary gland
Downregulation of runt‐related transcription factor 3 associated with poor prognosis of adenoid cystic and mucoepidermoid carcinomas of the salivary gland
复制标题
runt相关转录因子3的下调与唾液腺腺样囊性癌和粘液表皮样癌的不良预后相关
DOI：
10.1111/j.1349-7006.2010.01787.x
复制
发表时间：
2011
期刊：
影响因子：
5.7
通讯作者：
H. Kuniyasu
中科院分区：
文献类型：
--
作者：
T. Sasahira;Miyako Kurihara;Kazuhiko Yamamoto;U. Bhawal;T. Kirita;H. Kuniyasu
矮小相关转录因子3(RUNX3)是转化生长因子-β超家族的一种转录因子，是一种肿瘤抑制基因，在多种癌症中启动子区的高甲基化使其失活。本研究检测了RUNX3在涎腺多形性腺瘤(PA)、腺样囊性癌(ACC)和粘液表皮样癌(MEC)中的表达及甲基化状态。RUNX3在PA、ACC和MEC的胞浆阳性表达率分别为65%(13/20)、22.2%(8/36)和20.6%(7/34)。RUNX3在ACC和MEC中低表达或缺失与肿瘤进展和预后不良显著相关。显微切割后发现，RUNX3基因在涎腺腺样囊性癌和涎腺组织中的表达均低于正常涎腺组织和正常涎腺组织，且在PA(2/8，25%)、ACC(6/8，75%)和MEC(7/8，87.5%)组织中RUNX3基因甲基化的发生率明显高于非癌涎腺组织(0/8，0%)。我们的结果提示，RUNX3在涎腺肿瘤中的低表达或缺失可能在涎腺ACC和MEC的发生、发展和预后不良中起关键作用。RUNX3抑瘤功能的恢复可能抑制人唾液腺的肿瘤发生和肿瘤进展。(《癌症科学》2011；102：492-497)
Runt‐related transcription factor 3 (RUNX3) is a transcription factor of the transforming growth factor (TGF)‐β superfamily and acts as a tumor suppressor gene, which is silenced by hypermethylation of the promoter region in various cancers. In this study, we examined the expression and methylation status of RUNX3 in the salivary gland cancers pleomorphic adenoma (PA), adenoid cystic carcinoma (ACC) and mucoepidermoid carcinoma (MEC). The cytoplasmic expression rates of RUNX3 in PA, ACC and MEC were 65% (13/20), 22.2% (8/36) and 20.6% (7/34), respectively. Low expression or deletion of RUNX3 in ACC and MEC was significantly associated with tumor progression and poor prognosis. Using microdissected cDNA, we found that RUNX3 mRNA expression was lower in ACC and MEC than in PA and noncancerous salivary glands; furthermore, hypermethylation of RUNX3 was detected more frequently in PA (2/8, 25%), ACC (6/8, 75%) and MEC (7/8, 87.5%) than in noncancerous salivary glands (0/8, 0%). Our results suggest that low expression or deletion of RUNX3 in salivary gland tumors might play a pivotal role in tumorigenesis and tumor progression and poor prognosis in the case of salivary gland ACC and MEC. Recovery of the tumor suppressive function of RUNX3 might inhibit tumorigenesis and cancer progression in the human salivary gland. (Cancer Sci 2011; 102: 492–497)
影响因子：
11.2
作者：
G. Barnes;A. Javed;Sylvan M Waller;M. Kamal;Kerri E Hebert;Mohammad Q. Hassan;A. Bellahcène;A. V.
通讯作者：
G. Barnes;A. Javed;Sylvan M Waller;M. Kamal;Kerri E Hebert;Mohammad Q. Hassan;A. Bellahcène;A. V.