SITES OF SERUM ALPHA-FETOPROTEIN SYNTHESIS IN HUMAN AND IN RAT
SITES OF SERUM ALPHA-FETOPROTEIN SYNTHESIS IN HUMAN AND IN RAT
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DOI：
10.1172/jci105590
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发表时间：
1967-01-01
影响因子：
15.9
通讯作者：
BOESMAN, M
中科院分区：
文献类型：
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作者：
GITLIN, D;BOESMAN, M
将来自妊娠6至9周的人胚胎、妊娠15天的大鼠胎儿和2天龄大鼠的选定组织与14 C标记的氨基酸孵育。孵育后，使用兔抗人和大鼠胎血清蛋白的抗血清对培养液进行免疫电泳，然后进行放射自显影，结果显示：1）人肝、大鼠肝和大鼠卵黄囊培养物中存在放射性[α]-甲胎蛋白，但人或大鼠脑、肺、心脏、肾、肠、骨骼肌、皮肤或胎盘培养物中不存在放射性甲胎蛋白;未研究人卵黄囊。2)放射性转铁蛋白也存在于大鼠卵黄囊培养物中，在大鼠肝培养物中也发现了相同的蛋白质。3)大鼠肝脏和大鼠胎盘培养物均产生放射性血清Ra 2-球蛋白。出生后，大鼠血清甲胎蛋白浓度突然下降，到2 - 3日龄时约为产前水平的一半，这与分娩时胎膜的丢失是雅阁的;然后甲胎蛋白水平保持相对恒定，直到大鼠6 - 8日龄，此后蛋白质的合成受到越来越多的抑制。
Selected tissues from human embryos of 6 to 9 weeks'' gestation, from rat fetuses of 15 days'' gestation, and from rats 2 days of age were incubated with 14c-labeled amino acids. Immunoelectrophoresis of the culture fluid after incubation, using rabbit antisera against human and rat fetal serum proteins, followed by radioautography revealed that: 1) Radioactive [alpha]-fetoprotein was present in cultures of human liver, rat liver, and rat yolk sac, but not in cultures of human or rat brain, lung, heart, kidney, intestines, skeletal muscle, skin, or placenta; human yolk sac was not studied. 2) Radioactive transferrin was also present in rat yolk sac cultures, and the same protein was found in rat liver cultures as well. 3) Rat liver and rat placenta cultures both produced radioactive serum Ra2-globulin. Serum a-fetoprotein concentrations in the rat declined abruptly after birth to approximately half of the prenatal level by 2 to 3 days of age, in accord with the loss of the fetal membranes at delivery; the a-fetoprotein level then remained relatively constant until the rat was 6 to 8 days of age, after which synthesis of the protein was increasingly suppressed.