Construction of a Microstructured Collagen Membrane Mimicking the Papillary Dermis Architecture and Guiding Keratinocyte Morphology and Gene Expression
Construction of a Microstructured Collagen Membrane Mimicking the Papillary Dermis Architecture and Guiding Keratinocyte Morphology and Gene Expression
复制标题
DOI：
10.1002/mabi.201100443
复制
发表时间：
2012-05-01
影响因子：
4.6
通讯作者：
van Kuppevelt, Toin H.
中科院分区：
文献类型：
--
作者：
Lammers, Gerwen;Roth, Guenter;van Kuppevelt, Toin H.
创建乳头状结构的胶原纤维膜，模仿人类乳头状真皮的 3D 结构。将在乳头结构膜上培养至汇合的原代人角质形成细胞与在平坦膜上培养的角质形成细胞进行比较。显微镜评估揭示了乳头结构底部存在形态不同的细胞，而在平膜上观察不到这些细胞。基因表达微阵列和 RT-qPCR 表明这些细胞处于更加增殖/迁移的状态，而平膜上的细胞具有更加分化的表达谱。免疫组织化学染色证实了这些结果。总之，特定的胶原蛋白结构可以指导角质形成细胞的行为，这可用于进一步改善皮肤再生。
A papillary-structured collagen fibril membrane is created, mimicking the 3D-architecture of the human papillary dermis. Primary human keratinocytes cultured to confluency on papillar-structured films are compared to keratinocytes cultured on flat membranes. Microscopical evaluation reveals the presence of morphologically distinct cells at the base of the papillar structures that are not observed on flat membranes. Gene expression microarrays and RT-qPCR indicate that these cells are in a more proliferative/migrational state, whereas cells on flat membranes have a more differentiated expression profile. Immunohistochemical stainings confirm these results. In conclusion, specific collagen architecture can direct keratinocyte behavior, and this may be used to further improve skin regeneration.