Immunohistological study of the immune system cells in paraffin-embedded tissues of conventional pigs
Immunohistological study of the immune system cells in paraffin-embedded tissues of conventional pigs
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DOI：
10.1016/s0165-2427(01)00364-6
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发表时间：
2001-10-01
影响因子：
1.8
通讯作者：
Domingo, M
中科院分区：
文献类型：
--
作者：
Chianini, F;Majó, N;Domingo, M
采用免疫组织学技术，在福尔马林固定的常规饲养的健康猪石蜡包埋组织中研究了免疫系统不同细胞的分布。采集的样本为：肺、扁桃体、淋巴结（纵隔、肠系膜、腹股沟和下颌下）、胰腺、脾、肝、肾、肾上腺、回肠和胃。采用标准抗生物素蛋白-生物素过氧化物酶（ABC）方法，共使用6种一抗抗-CD 3、抗-CD 79 α、Mac 387、抗-溶菌酶、抗-CD 45 RA（3C 3/9）和抗-SLA-II-DQ（BL 2 H5）。抗-CD 3和抗-CD 79 α mAb分别与位于T细胞区和B细胞区的细胞反应。Mac 387识别循环多形核白细胞，而抗溶菌酶染色的所有组织中的常驻巨噬细胞。首次在福尔马林固定的石蜡包埋组织中评估了3C 3/9和BL 2 H5。3C 3/9在初级卵泡和套层区鉴定出B淋巴细胞，T细胞亚群，特别是位于脾脏边缘区和生殖中心的可变数量的免疫母细胞。BL 2 H 5与淋巴组织滤泡外套带中的B细胞反应，与所有研究的淋巴组织中的树突状细胞和叉指状细胞反应，并与脾的动脉周围淋巴鞘（帕尔斯）、边缘区和红髓中不同数量的静息和活化T细胞反应。此外，它还对肝脏中的枯否细胞和血管周围巨噬细胞进行了染色。本研究对常规健康猪中最重要的免疫系统细胞亚群的分布进行了详细的组织学研究。在我们看来，这些工具应该是有益的，为未来的疾病状况的比较研究。(C)2001 Elsevier Science B. V.保留所有权利。
The distribution of different cells of the immune system has been studied in formalin-fixed paraffin-embedded tissues from conventionally reared healthy pigs, using immunohistological techniques. The samples collected were: lungs, tonsils, lymph nodes (mediastinal, mesenteric, inguinal and submandibular), pancreas, spleen, liver, kidney, adrenal gland, ileum and stomach. A total of six primary antibodies anti-CD3, anti-CD79 alpha, Mac 387, anti-lysozyme, anti-CD45RA (3C3/9) and anti-SLA-II-DQ (BL2H5) were used with a standard avidin-biotin peroxidase (ABC) method. Anti-CD3 and anti-CD79 alpha mAb-reacted, respectively with cells located in T cell areas and B cell areas. Mac 387 recognised circulating polymorphonuclear leukocytes, while anti-lysozyme-stained resident macrophages in all tissues. 3C3/9 and BL2H5, were assessed in formalin-fixed paraffin-embedded tissues for the first time. 3C3/9 identified B lymphocytes, in primary follicles and mantle zones, a subpopulation of T cells, especially located in the marginal zone of the spleen and a variable number of immunoblasts, in the germinal centres. BL2H5 reacted with B cells in the mantle zones of the follicles of lymphoid tissues, with dendritic and interdigitating cells in all studied lymphoid tissues and With a variable number of resting and activated T cells in the periarteriolar lymphoid sheath (PALs), marginal zone and red pulp of the spleen. Furthermore, it stained Kupffer and perivascular macrophages in the liver This study represents a detailed histological study of the distribution of the most important subpopulations of immune system cells in conventional, healthy pigs. In our view, these tools should be useful for future comparative studies in disease conditions. (C) 2001 Elsevier Science B.V. All rights reserved.