THE NUCLEAR 5S RNAS FROM CHICKEN, RAT AND MAN - U5 RNAS ARE ENCODED BY MULTIPLE GENES
THE NUCLEAR 5S RNAS FROM CHICKEN, RAT AND MAN - U5 RNAS ARE ENCODED BY MULTIPLE GENES
复制标题
DOI：
10.1093/nar/9.4.769
复制
发表时间：
1981-01-01
影响因子：
14.9
通讯作者：
BRANLANT, C
中科院分区：
文献类型：
--
作者：
KROL, A;GALLINARO, H;BRANLANT, C
鸡、大鼠和人类核5S RNA的制备物含有两组分子。具有最低电泳迁移率（5Sa）的组包含与来自相应动物物种的核糖体5S RNA相同或密切相关的RNA。在HeLa细胞和大鼠脑中，我们只检测到与核糖体5S RNA相同的RNA。在母鸡的大脑和肝脏中，我们发现其他物种的不同之处在于有限数量的替代。结果表明，突变的5S基因可能根据细胞类型而不同地表达。具有最高迁移率的集合对应于U 5 RNA。在大鼠脑和HeLa细胞中，发现U 5 RNA分别由4种和5种不同的分子（U 5 A、U 5 B1 -4）组成，不同之处在于少量的取代或插入。在鸡脑中，未检测到U 5 B，但U 5A ′与U 5A不同，其3′-末端腺苷缺失。所有U 5 RNA都含有相同的修饰核苷酸组。它们也具有相同的二级结构，由两个发夹通过17个核苷酸长的单链区域连接在一起组成。分子的3′半具有紧密构象。总之，这些结果表明，U 5 RNA是从一个多基因家族转录的，只要二级结构保守，突变基因就可能表达。U 5 RNA的构象可能与其功能有关，值得一提的是，在U 5和U1 A RNA之间发现了一些结构相似性。
Preparations of chicken, rat and human nuclear 5S RNA contain two sets of molecules. The set with the lowest electrophoretic mobility (5Sa) contains RNAs identical or closely related to ribosomal 5S RNA from the corresponding animal species. In HeLa cells and rat brain, we only detected an RNA identical to the ribosomal 5S RNA. In hen brain and liver, we found other species differing by a limited number of substitutions. The results suggest that mutated 5S genes may be expressed differently according to the cell type. The set with the highest mobility corresponds to U5 RNA. In both rat brain and HeLa cells, U5 RNA was found to be composed of 4 and 5 different molecules respectively (U5A, U5B1–4) differing by a small number of substitutions or insertions. In hen brain, no U5B was detected but U5A′ differing from U5A by the absence of the 3′-terminal adenosine. All the U5 RNAs contain the same set of modified nucleotides. They also have the same secondary structure which consists of two hairpins joined together by a 17 nucleotide long single-stranded region. The 3′ half of the molecule has a compact conformation. Together, the results suggest that U5 RNAs are transcribed from a multigene family and that mutated genes may be expressed as far as secondary structure is conserved. The conformation of U5 RNA is likely to be related to its function and it is of interest to mention that several similarities of structure are found between U5 and U1A RNA.