Water quality, Vibrio density and growth of Pacific white shrimp Litopenaeus vannamei (Boone) in an integrated biofloc system with red seaweed Gracilaria birdiae (Greville)
Water quality, Vibrio density and growth of Pacific white shrimp Litopenaeus vannamei (Boone) in an integrated biofloc system with red seaweed Gracilaria birdiae (Greville)
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DOI：
10.1111/are.12552
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发表时间：
2016-03
影响因子：
2
通讯作者：
L. O. Brito;Augusto Monteiro Chagas;Elizabeth Pereira da Silva;R. Soares;W. Severi;A. Gálvez
中科院分区：
文献类型：
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作者：
L. O. Brito;Augusto Monteiro Chagas;Elizabeth Pereira da Silva;R. Soares;W. Severi;A. Gálvez
对南美白对虾进行了为期42天的室内试验，评价了南美白对虾的水质、弧菌密度和生长情况。试验共设4个处理，每组3个：对照组(单养对虾)、IBS2.5(凡纳滨对虾+2.5 kg湿重海藻m−3)、IBS5.0(凡纳滨对虾+5 kg湿重海藻m−3)和IBS7.5(凡纳滨对虾+7.5 kg湿重海藻m−3)。对虾个体(0.34±0.0 1g)按5 0 0 m m−3密度放养。以糖蜜为有机碳源，每天添加一次糖蜜，使碳氮比维持在12：1。溶解无机氮(DIN)19%~34%(3.12~3.83 mg L−1)，硝态氮19%~38%(2.40~3.16 mg L−1)，弧菌密度8~83%(0.40~2.20log 103菌落形成单位毫升−1)，FCR20~30%(1.2~1.37)，与对照组(L−1 4.73mg，L−1 3.93 mg)相比，2.40log 10~3个集落形成单位m L−1和1.74)。此外，添加IBS显著提高了(P<；0.05)对虾的粗蛋白含量，变化幅度为8%~13%(湿重13.2%~13.7%)；与对照组(湿重分别为12.1%、3.12g、0.47g和1.41kgm−3)相比，平均每周增重率为25%~34%(0.59~0.63g)，对虾产量提高22~39%(1.72~1.96 kg m−3)。因此，用南美白对虾在IBS中培养江豚，可以降低弧菌密度，提高对虾体内的粗蛋白质含量，提高对虾的生长和产量。
An indoor trial was conducted for 42 days to evaluate water quality, Vibrio density and growth of Litopenaeus vannamei in an integrated biofloc system (IBS) with Gracilaria birdiae. Four treatments were used, each in triplicate: Control (monoculture shrimp); IBS 2.5 (L. vannamei and 2.5 kg wet weight seaweed m−3); IBS 5.0 (L. vannamei and 5.0 kg wet weight seaweed m−3) and IBS 7.5 (L. vannamei and 7.5 wet weight seaweed m−3). Shrimp individuals (0.34 ± 0.01 g) were stocked at a density of 500 shrimp m−3. No water exchange was carried out during the experimental period. Molasses was added once a day as an organic carbon source to maintain the C:N ratio at 12:1. The IBS significantly decreased (P < 0.05) dissolved inorganic nitrogen (DIN) ranging from 19% to 34% (3.12–3.83 mg L−1), NO3-N ranging from 19% to 38% (2.40–3.16 mg L−1), Vibrio density ranging from by 8–83% (0.40–2.20 log 103 colony-forming units mL−1), and FCR ranging from by 20–30% (1.20–1.37), as compared to the control (4.73 mg L−1, 3.93 mg L−1, 2.40 log 103 colony-forming units mL−1, and 1.74 respectively). Moreover, the IBS significantly increased (P < 0.05) crude protein in whole body shrimp, ranging from 8% to 13% (13.2–13.7% wet weight basis); as well as final weight, ranging from 25% to 32% (3.90–4.12 g), weekly growth ranging from 25% to 34% (0.59–0.63 g), and shrimp yield by 22–39% (1.72–1.96 kg m−3), as compared to control (12.1% wet weight basis, 3.12 g, 0.47 g, and 1.41 kg m−3 respectively). It can thus be concluded that cultivating Gracilaria birdiae in an IBS with L. vannamei can contribute to DIN and NO3-N removal, lower Vibrio density, increased crude protein in whole body shrimp, higher growth and yield parameters in shrimp culture.