Dendritic compartmentation of NMDA receptor mRNA in cultured hippocampal neurons
Dendritic compartmentation of NMDA receptor mRNA in cultured hippocampal neurons
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DOI：
10.1097/00001756-199703030-00004
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发表时间：
1997-03-03
期刊：
影响因子：
1.7
通讯作者：
Benson, DL
中科院分区：
文献类型：
--
作者：
Benson, DL
用原位杂交法检测了N-甲基-D-天冬氨酸(NMDAR1)受体复合体必需亚基(NMDAR1)编码基因在海马神经元的细胞内定位。从树突开始发育的时候，NMDAR1mRNA的标记就从细胞体细胞延伸到早期的树突。培养10d时，微管蛋白相关蛋白2mRNA的分布模式与微管蛋白相关蛋白2mRNA的分布模式相似，但与GluR1或β-肌动蛋白mRNAs的分布模式形成鲜明对比，两者均局限于细胞胞体。NMDAR1基因在星形胶质细胞中也有表达，广泛分布于细胞质中。这些数据表明，NMDAR1mRNA的转运可能是靶向和操纵NMDA受体局部浓度的机制。
THE intracellular localization of mRNA encoding the obligatory subunit for the N-methyl-D-aspartate (NMDA) receptor complex (NMDAR1) was determined in cultured hippocampal neurons by in situ hybridization. From the time at which dendrites begin to develop, labelling for NMDAR1 mRNA extended beyond cell somata into incipient dendritic processes. By 10 days in culture, label was found throughout dendritic arbors in a distribution pattern similar to that for microtublin associated proteins 2 mRNA, but contrasted sharply with that for GluR1 or beta-actin mRNAs, both of which were restricted to cell somata. NMDAR1 mRNA was also expressed in astrocytes, where it was diffusely localized throughout the cytoplasm. These data suggest that transport of NMDAR1 mRNA may serve as a mechanism to target and manipulate local concentrations of NMDA receptors.