Sinorhizobium fredii HH103 syrM inactivation affects the expression of a large number of genes, impairs nodulation with soybean and extends the host-range to Lotus japonicus
Sinorhizobium fredii HH103 syrM inactivation affects the expression of a large number of genes, impairs nodulation with soybean and extends the host-range to Lotus japonicus
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DOI：
10.1111/1462-2920.14897
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发表时间：
2020-03-01
影响因子：
5.1
通讯作者：
Vinardell, Jose-Maria
中科院分区：
文献类型：
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作者：
Acosta-Jurado, Sebastian;Alias-Villegas, Cynthia;Vinardell, Jose-Maria
Sinorhizobium fredii HH103 Rif (R) 是一种宿主范围广泛的根瘤菌菌株，能够与大豆和百脉根结瘤，但对日本乳杆菌无效。在这里，我们研究了 HH103 Rif (R) SyrM 蛋白在基因表达调节中的作用及其与这三种豆类共生的相关性。 RNAseq 分析表明，HH103 SyrM 不仅在诱导类黄酮存在时而且在诱导类黄酮不存在时都是重要的转录调节因子。缺乏SyrM会增加HH103中Nod因子的产生并减少金雀异黄素介导的胞外多糖产生的抑制。在共生中，syrM 的突变部分损害了与大豆的相互作用，但提高了与 L. burttii 的有效性，并将宿主范围扩大到 L. japonicus Gifu。此外，HH103 syrM 突变体通过感染线进入两种莲花物种，而 HH103 使用更原始的细胞间感染进入 L. burttii 根。这些共生表型之前在受共生调节因子 nodD2 和 nolR 影响的另外两个 HH103 突变体中观察到，揭示了 S. fredii HH103 中许多转录调节因子 调节共生基因表达。
Sinorhizobium fredii HH103 Rif (R) is a broad host-range rhizobial strain able to nodulate with soybean and Lotus burttii, but it is ineffective with L. japonicus. Here, we study the role of the HH103 Rif (R) SyrM protein in the regulation of gene expression and its relevance in symbiosis with those three legumes. RNAseq analyses show that HH103 SyrM is an important transcriptional regulator not only in the presence of inducer flavonoids but also in its absence. Lack of SyrM increases Nod factors production and decreases genistein-mediated repression of exopolysaccharide production in HH103. In symbiosis, mutation of syrM partially impaired interaction with soybean but improves effectiveness with L. burttii and extends the host-rage to L. japonicus Gifu. In addition, HH103 syrM mutants enter in both Lotus species by infection threads, whereas HH103 uses the more primitive intercellular infection to enter into L. burttii roots These symbiotic phenotypes were previously observed in two other HH103 mutants affected in symbiotic regulators, nodD2 and nolR, revealing that in S. fredii HH103 numerous transcriptional regulators finely modulate symbiotic gene expression.