PLANAR GLASS CHIPS FOR CAPILLARY ELECTROPHORESIS - REPETITIVE SAMPLE INJECTION, QUANTITATION, AND SEPARATION EFFICIENCY
PLANAR GLASS CHIPS FOR CAPILLARY ELECTROPHORESIS - REPETITIVE SAMPLE INJECTION, QUANTITATION, AND SEPARATION EFFICIENCY
复制标题
DOI：
10.1021/ac00058a029
复制
发表时间：
1993-05-15
影响因子：
7.4
通讯作者：
MANZ, A
中科院分区：
文献类型：
--
作者：
SEILER, K;HARRISON, DJ;MANZ, A
介绍了一种集成在平板玻璃上的样品进样系统和毛细管电泳通道，并对其性能进行了评价。至少25 kV的电压可以施加在11 cm长的毛细管通道上，产生的焦耳热充分耗散（1.8 W/m）。玻璃基板承受至少10（5）V/cm的直流电而不发生介电击穿。使用激光诱导荧光检测，荧光素衍生物和异硫氰酸荧光素标记的氨基酸的混合物被注射和分离。在优化条件下获得了高达100 000个理论塔板和约20个塔板/V，与熔融石英毛细管的结果相当。定量分析表明，对于长度大于200 μ m的注射塞，峰面积与注射量成正比。在0.85 cm长的分离通道中存在两个矩形角并没有可检测地增加分散，这表明可以在小于1 cm 2的面积中制造长度为0.5 m的蛇形毛细管通道。
A sample injection system and a capillary electrophoresis channel integrated together on a planar glass substrate are described, and the performance is evaluated. Voltages of at least 25 kV may be applied over an 11-cm-long capillary channel with sufficient dissipation of the Joule heat generated (1.8 W/m). The glass substrate sustains at least 10(5) V/cm dc without dielectric breakdown. Using laser-induced fluorescence detection, mixtures of fluorescein derivatives and fluorescein isothiocyanate-labeled amino acids were injected and separated. Up to 100 000 theoretical plates and approximately 20 plates/V were obtained under optimized conditions, comparable to results with fused-silica capillaries. Quantitative analysis showed that peak areas were proportional to the amount injected for injected plug lengths greater than 200 mum. The presence of two rectangular corners in a 0.85-cm-long separation channel did not increase dispersion detectably, indicating a serpentine capillary channel 0.5 m in length could be fabricated in less than a 1-cm2 area.