Recovery of Lutein from Microalgae Biomass: Development of a Process for Scenedesmus almeriensis Biomass
Recovery of Lutein from Microalgae Biomass: Development of a Process for Scenedesmus almeriensis Biomass
复制标题
DOI：
10.1021/jf8025875
复制
发表时间：
2008-12-24
影响因子：
6.1
通讯作者：
Fernandez-Sevilla, Jose M.
中科院分区：
文献类型：
--
作者：
Ceron, M. Carmen;Campos, Inmaculada;Fernandez-Sevilla, Jose M.
本文提出了一种从微藻生物量中提取叶黄素的优化方法。它是利用富含叶黄素的微藻（Scenedesmus almeriensis）的干生物量开发的。该方法包括三个步骤，细胞破坏、碱性处理和溶剂提取，并获得富含叶黄素的类胡萝卜素提取物。结果表明，细胞破坏是必要的，并且在工业应用方面测试的处理中，最佳选择是使用含有氧化铝的球磨机，以1:1 w/w的比例作为崩解剂5分钟。碱法处理的最佳条件为：KOH浓度为4% w/v，生物量浓度为100 g/L，碱法处理5 min。较长时间的碱性处理或使用较高的KOH浓度降低了该工艺的产率。最后对正己烷萃取工艺进行了优化。采用正己烷与样品体积1:1的比例，总共需要8个提取步骤，以回收处理后的生物质中含有的99%的叶黄素。最佳提取步骤为6步，叶黄素回收率为95%。综上所述，所开发的方法可以有效地从微藻生物量中回收叶黄素，是一种可扩展和工业应用的方法。
In this work an optimized method for the extraction of lutein from microalgae biomass is presented. It has been developed using dry biomass of the lutein-rich microalga Scenedesmus almeriensis. The method comprises three steps, cell disruption, alkaline treatment, and solvent extraction, and renders a carotenoid extract rich in lutein. The results demonstrate that cell disruption is necessary and that the best option among the treatments tested with regard to industrial applications is the use of a bead mill with alumina in a 1:1 w/w proportion as disintegrating agent for 5 min. With regard to the alkaline treatment, the optimal conditions were obtained using 4% w/v KOH with a biomass concentration of 100 g/L for 5 min. Longer alkaline treatments or the use of higher KOH concentrations reduced the yield of the process. Finally, extraction with hexane is optimized. Using a 1:1 ratio hexane to sample volume, a total of eight extraction steps are necessary to recover 99% of lutein contained in the processed biomass. However, the optimal number of extraction steps is six, 95% of the lutein being recovered. In summary, the developed method allows the efficient recovery of lutein from microalgae biomass, it being a scaleable and industrially applicable method.