How RNA folds
How RNA folds
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DOI：
10.1006/jmbi.1999.3001
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发表时间：
1999-10-22
影响因子：
5.6
通讯作者：
Bustamante, C
中科院分区：
文献类型：
--
作者：
Tinoco, I;Bustamante, C
我们描述了RNA折叠问题，并将其与更困难的蛋白质折叠问题进行了对比。RNA有四个相似的单体单元，而蛋白质有20个非常不同的残基。RNA的折叠是分层的，二级结构比三级折叠稳定得多。在RNA中，二级和三级折叠可以通过Mg2+的存在或不存在进行实验分离。从二级结构元素：螺旋、环和凸起的实验热力学数据可以成功地预测二级结构。然后可以添加三级相互作用，而不会对二级结构造成很大的扭曲。这些观察结果提出了一种折叠算法，可以根据RNA的序列预测其结构。然而，为了解决RNA折叠问题，我们需要三级结构相互作用的热力学数据，以及金属离子结合位点的识别和表征。这些数据，连同对单个RNA分子的力与延伸的测量，应该为测试和完善所提出的算法提供必要的信息。(C) 1999学术出版社。
We describe the RNA folding problem and contrast it with the much more difficult protein folding problem. RNA has four similar monomer units, whereas proteins have 20 very different residues. The folding of RNA is hierarchical in that secondary structure is much more stable than tertiary folding. In RNA the two levels of folding (secondary and tertiary) can be experimentally separated by the presence or absence of Mg2+. Secondary structure can be predicted successfully from experimental thermodynamic data on secondary structure elements: helices, loops, and bulges. Tertiary interactions:can :then be added without much distortion of the secondary structure. These observations suggest a folding algorithm to predict the structure of an RNA from its sequence. However, to solve the RNA folding problem one needs thermodynamic data on tertiary structure interactions, and identification and characterization of metal-ion binding sites. These data, together with force versus extension measurements on single RNA molecules, should provide the information necessary to test and refine the proposed algorithm. (C) 1999 Academic Press.