Multiple opiate receptor subtypes are involved in the stimulation of growth hormone release by beta-endorphin in female rats.
Multiple opiate receptor subtypes are involved in the stimulation of growth hormone release by beta-endorphin in female rats.
复制标题
多种阿片受体亚型参与雌性大鼠体内β-内啡肽刺激生长激素的释放。
DOI：
10.1159/000126721
复制
发表时间：
1994
影响因子：
4.1
通讯作者：
Callahan,P
中科院分区：
文献类型：
--
作者：
Janik,J;Klosterman,S;Parman,R;Callahan,P
在间情期雌性大鼠，观察了生长激素（GH）对β-内啡肽的分泌反应及阿片受体亚型参与GH分泌反应的情况。通过在β-内啡肽之前给予这些位点中的每一个的特异性拮抗剂来确定mu（μ）、delta（δ）和/或kappa（K）位点的参与。给予β-Funaltamine（1或5 μg）阻断μ位点，ICI 154，129（5或25 μg）阻断δ位点，nor-binaltorphimine（8 μg）阻断K位点。还测定了这些拮抗剂在静脉注射吗啡后阻断GH分泌的能力。将阿片受体拮抗剂和β-内啡肽注入侧脑室。β-内啡肽的剂量反应研究表明，0.5 µg β-内啡肽是GH释放的最小刺激剂量，使循环GH水平增加约4倍;较低剂量的β-内啡肽不会刺激分泌。所有三种拮抗剂都能够阻断β-内啡肽的刺激作用。这些结果提供了证据表明，所有三种阿片受体亚型都参与了β-内啡肽对GH释放的刺激作用。
The growth hormone (GH) secretory response to β-endorphin and the involvement of opiate receptor subtypes in this response were determined in diestrous female rats. The involvement of the mu (µ), delta (δ) and/or kappa (K) site was determined by administering specific antagonists for each of these sites prior to β-endorphin. β-Funaltrexamine (1 or 5 µg) was administered to block µ sites, ICI 154,129 (5 or 25 µg) blocked δ sites and nor-binaltorphimine (8 µg) blockedKsites. The ability of these antagonists to block GH secretion following intravenous morphine administration was also determined. The opiate antagonists and β-endorphin were administered into the lateral ventricle. A dose-response study for β-endorphin indicated that 0.5 µg β-endorphin was the minimum stimulatory dose for GH release, producing an approximately 4-fold increase in circulating levels of GH; lower doses of β-endorphin did not stimulate secretion. All three antagonists were capable of blocking the stimulatory effects of β-endorphin. These results provide evidence that all three opiate receptor subtypes are involved in the stimulatory effect of β-endorphin on GH release.