STUDIES ON THE ADENOSINE TRIPHOSPHATE-PHOSPHATE EXCHANGE AND THE HYDROLYSIS OF ADENOSINE TRIPHOSPHATE CATALYSED BY A PARTICULATE FRACTION FROM THE MOSQUITO
STUDIES ON THE ADENOSINE TRIPHOSPHATE-PHOSPHATE EXCHANGE AND THE HYDROLYSIS OF ADENOSINE TRIPHOSPHATE CATALYSED BY A PARTICULATE FRACTION FROM THE MOSQUITO
复制标题
DOI：
10.1042/bj0720008
复制
发表时间：
1959-01-01
影响因子：
4.1
通讯作者：
GONDA, O
中科院分区：
文献类型：
--
作者：
AVIDOR, Y;GONDA, O
在埃及伊蚊（Aedes aegypti L.）制备的呼吸道颗粒中研究了腺苷三磷酸酶和催化P32进入三磷酸腺苷的酶系统。在分离培养基中加入乙二胺四乙酸，在洗涤液或测定培养基中加入乙二胺四乙酸和白蛋白是交换反应的必要条件。研究了核苷酸、呼吸抑制剂和氧化磷酸化抑制剂对交换反应和三磷酸腺苷活性的影响。昆虫和哺乳动物呼吸微粒对1,1,1 -三氯- 2,2 -二-（对氯苯）-乙烷（DDT）的反应存在数量上的差异。0. 1 mM DDT对昆虫肌小体交换反应的抑制作用大于50%，而对哺乳动物肝脏线粒体的抑制作用小于10%。
Adenosine triphosphatase and the enzyme system catalyzing the incorporation of P32 into adenosine triphosphate have been studied in respiratory particles prepared from the mosquito Aedes aegypti L. Addition of ethylenediaminetetra-acetic acid to the isolation medium and of ethylenediaminetetra-acetic acid and albumin either to the washing liquid or to the assay medium has been found obligatory for the exchange reaction. The effect of nucleotides, respiratory inhibitors and inhibitors of oxidative phosphorylation on the exchange reaction and on the adenosine triphosphate activity has been investigated. Quantitative differences exist between the response of insect and mammalian respiratory particles to 1, 1, 1-trichloro-2, 2-di-(p-chlorophenyl)-ethane (DDT). 0. 1 mM DDT inhibits the exchange reaction by more than 50% in insect sarcosomes whereas the inhibition in mammalian liver mitochondria is less than 10%.