Estimation of the distribution of intravenously injected adipose tissue-derived stem cells labeled with quantum dots in mice organs through the determination of their metallic components by ICPMS.
Estimation of the distribution of intravenously injected adipose tissue-derived stem cells labeled with quantum dots in mice organs through the determination of their metallic components by ICPMS.
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DOI：
10.1021/ac202053y
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发表时间：
2011-10
影响因子：
7.4
通讯作者：
Y. Takasaki;Masaki Watanabe;H. Yukawa;A. Sabarudin;K. Inagaki;N. Kaji;Y. Okamoto;M. Tokeshi;Y. Miyamoto;H. Noguchi;T. Umemura;S. Hayashi;Y. Baba;H. Haraguchi
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文献类型：
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作者：
Y. Takasaki;Masaki Watanabe;H. Yukawa;A. Sabarudin;K. Inagaki;N. Kaji;Y. Okamoto;M. Tokeshi;Y. Miyamoto;H. Noguchi;T. Umemura;S. Hayashi;Y. Baba;H. Haraguchi
脂肪组织源性干细胞（ASCs）由于其自我更新受损或病变器官的能力和易于收获而在细胞治疗中显示出前景。为了保证尾静脉注射ASCs的分布和定量，包括量子点荧光光学成像（QDs）在内的几种全身成像技术已经被采用，但它们的灵敏度和准确性都不够。本文通过电感耦合等离子体质谱（ICPMS）检测qds衍生金属成分，报道了静脉注射qds标记的ASCs （QDs-ASCs）小鼠各器官（心、肺、肝、脾和肾）中ASCs的定量分布。为了准确和精确地测定，在ICPMS测量之前，每个器官都被采集并在微波炉中用HNO(3)和H(2)O(2)的混合物完全消化，配备了微流注射系统和实验室自制的毛细管附着式微雾化器。优化后，成功测定了16种元素，包括量子点的主成分（Cd、Se、Te）和必需元素（Na、K、Mg、Ca、P、S、Mn、Fe、Co、Cu、Zn、Se、Sr、Mo）。与未处理的小鼠相比，QDs- ascs处理的小鼠在所有器官中Cd和Te的水平均显著升高，并且正如预期的那样，各器官中Cd与Te的摩尔组成比与QDs中的摩尔组成比吻合良好。这表明Cd（或Te）的增加可以作为计算小鼠器官内ASCs分布的示踪剂。计算结果显示，估计总注射ASCs中36.8%、19.1%、0.59%、0.49%和0.25%分别分布在肝脏、肺、心脏、脾脏和肾脏。
Adipose tissue-derived stem cells (ASCs) have shown promise in cell therapy because of their ability to self-renew damaged or diseased organs and easy harvest. To ensure the distribution and quantification of the ASCs injected from tail vein, several whole-body imaging techniques including fluorescence optical imaging with quantum dots (QDs) have been employed, but they may suffer from insufficient sensitivity and accuracy. Here, we report quantitative distribution of ASCs in various organs (heart, lung, liver, spleen, and kidney) of mice, which were intravenously injected with QDs-labeled ASCs (QDs-ASCs), through the detection of QDs-derived metallic components by inductively coupled plasma mass spectrometry (ICPMS). For accurate and precise determination, each organ was harvested and completely digested with a mixture of HNO(3) and H(2)O(2) in a microwave oven prior to ICPMS measurement, which was equipped with a microflow injection system and a laboratory-made capillary-attached micronebulizer. After optimization, 16 elements including major components (Cd, Se, and Te) of QDs and essential elements (Na, K, Mg, Ca, P, S, Mn, Fe, Co, Cu, Zn, Se, Sr, and Mo) were successfully determined in the organs. As compared to untreated mice, QDs-ASCs-treated mice showed significantly higher levels of Cd and Te in all organs, and as expected, the molar ratio of Cd to Te in each organ was in good agreement with the molar composition ratio in the QDs. This result indicates that the increment of Cd (or Te) can be used as a tracer for calculating the distribution of ASCs in mice organs. As a result of the calculation, 36.8%, 19.1%, 0.59%, 0.49%, and 0.25% of the total ASCs injected were estimated to be distributed in the liver, lung, heart, spleen, and kidney, respectively.