Antitumor effect of gold as revealed by growth suppression of cultured cancer cells
Antitumor effect of gold as revealed by growth suppression of cultured cancer cells
复制标题
DOI：
10.1089/cbr.1998.13.189
复制
发表时间：
1998-06-01
影响因子：
3.4
通讯作者：
Kamei, H
中科院分区：
文献类型：
--
作者：
Koide, T;Kojima, T;Kamei, H
金制剂已广泛应用于类风湿关节炎的治疗。我们在体外研究了这种药物对恶性细胞的生长抑制作用。用浓度为2 μ g/ml的金剂分别培养HCT-15、人恶性肿瘤细胞AGS和Balb/C小鼠恶性淋巴瘤细胞Meth/ a。在37℃、5% CO2的培养箱中培养4天后，进行细胞计数；采用Student’st检验分析差异的显著性。另外，用金培养HCT-15细胞2天，然后用流式细胞术分析细胞。金可抑制HCT-15、AGS和Meth/A细胞的生长。HCT-15细胞在50 μ g/ml ~ 10 μ g/ml、AGS细胞在125 μ g/ml ~ 50 μ g/ml、Meth/ a细胞在125 μ g/ml ~ 50 μ g/ml浓度范围内抑制50%。在50 μ g/ml和10 μ g/ml之间，顺铂对HCT-15细胞生长的抑制率为50%。流式细胞术结果显示，四倍体峰值显著升高，二倍体和四倍体峰值之间的差值轻微升高，倍体大于4的细胞增加。这些数据表明，金阻断了S相，G2到M相，以及M相。为了观察金的细胞毒性，10只4周龄Balb/C小鼠每隔一天注射10 mg/kg或2 mg/kg，连续3次注射，或每天注射30 mg/kg金，连续10天。观察20天后，所有小鼠仍然存活。10只小鼠每只注射顺铂10 mg/kg，每只注射1次，60%的小鼠在注射后10天内死亡。
Gold agents have been widely used for the treatment of rheumatoid arthritis. We studied the growth inhibiting effect of such an agent on malignant cells in vitro.HCT-15, AGS cells derived from a human malignancy, and Meth/A cells from a malignant lymphoma of Balb/C mice were cultured separately with gold agent at concentrations of 2 mu g/ml. Four days after the cultures had been incubated in a 5% CO2 incubator at 37 degrees C, cell counts were made; and significance of differences was analyzed by Student's t test. Additionally, HCT-15 cells were cultured with gold for two days, and then the cells were analyzed by flow cytometry.The growth of HCT-15, AGS, and Meth/A cells was suppressed by gold. Fifty percent suppression was observed at a concentration between 50 mu g/ml and 10 mu ug/ml for HCT-15 cells, between 125 mu ug/ml and 50 mu g/ml for AGS cells, and between 125 mu g/ml and 50 mu ug/ml for Meth/A cells. Fifty percent suppression of HCT-15 cell growth by cisplatinum was found between 50 mu g/ml and 10 mu g/ml.Flow cytometric findings showed a significant rise in the tetraploid peak, a mild rise in the resion between diploid and tetraploid peaks, and an increase in cells with a ploidy greater than four. These data suggest that gold blocks the S phase, G2 to M phase, and M phase as well.To observe the cytotoxicity of gold, each of 10 of 4 week-old Balb/C mice was injected s.c. at a dose of 10 mg/kg or 2 mg/kg every other day for a fetal of 3 injections, or was administered the gold at 30 mg/kg/day p.o. for 10 days. All mice were still alive after 20 days of observation. Cisplatinum at a dose of 10 mg/kg was also injected s.c. one time into each of 10 mice, and 60% of the animals died within 10 days after the injection.