Overexpression of AtWRKY28 and AtWRKY75 in Arabidopsis enhances resistance to oxalic acid and Sclerotinia sclerotiorum
Overexpression of AtWRKY28 and AtWRKY75 in Arabidopsis enhances resistance to oxalic acid and Sclerotinia sclerotiorum
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DOI：
10.1007/s00299-013-1469-3
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发表时间：
2013-10-01
影响因子：
6.2
通讯作者：
Lu, Guodong
中科院分区：
文献类型：
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作者：
Chen, Xiaoting;Liu, Jun;Lu, Guodong
基于拟南芥基因芯片，我们发现8个WRKY基因在草酸（Oxalic acid，OA）胁迫下表达上调，AtWRKY 28和AtWRKY 75过表达株系表现出对OA和核盘菌（Sclerotinia sclerotiorum）的抗性增强。草酸（Oxalic acid，OA）是核盘菌（Sclerotina sclerotiorum（S. sclerotiorum）和灰葡萄孢Botrytis cinerea（B. cinerea）。OA胁迫下差异表达基因的鉴定将有助于我们了解OA产生真菌在寄主植物中的致病机制，以及植物如何应对OA和病原菌感染的机制。基于拟南芥oligo基因芯片，我们发现8个WRKY基因在OA攻击后表达上调。过表达AtWRKY 28和AtWRK 75的拟南芥对OA和S.石蒜同时此外，我们的研究结果表明，AtWRKY 28和AtWRK 75的过表达诱导宿主植物的氧化爆发，抑制菌丝生长。sclerotiorum，从而抑制真菌感染。基因表达谱分析表明，AtWRKY 28和AtWRKY 75都是水杨酸（SA）和茉莉酸/乙烯（JA/ET）依赖的防御信号通路的转录调控因子，AtWRKY 28和AtWRKY 75主要激活JA/ET通路来防御S.菌核病和草酸胁迫。
Based on Arabidopsis microarray, we found 8 WRKY genes were up-regulated with Oxalic acid (OA) challenge, AtWRKY28 and AtWRKY75 overexpression lines showed enhanced resistance to OA and Sclerotinia sclerotiorum.The WRKY transcription factors are involved in various plant physiological processes and most remarkably in coping with diverse biotic and abiotic stresses. Oxalic acid (OA) is an important pathogenicity-determinant of necrotrophic phytopathogenic fungi, such as Sclerotina sclerotiorum (S. sclerotiorum) and Botrytis cinerea (B. cinerea). The identification of differentially expressed genes under OA stress should facilitate our understanding of the pathogenesis mechanism of OA-producing fungi in host plants, and the mechanism of how plants respond to OA and pathogen infection. Based on Arabidopsis oligo microarray, we found 8 WRKY genes that were up-regulated upon OA challenge. The Arabidopsis plants overexpressing AtWRKY28 and AtWRK75 showed enhanced resistance to OA and S. sclerotiorum simultaneously. Furthermore, our results showed that overexpression of AtWRKY28 and AtWRK75 induced oxidative burst in host plants, which suppressed the hyphal growth of S. sclerotiorum, and consequently inhibited fungal infection. Gene expression profiling indicates that both AtWRKY28 and AtWRKY75 are transcriptional regulators of salicylic acid (SA)- and jasmonic acid/ethylene (JA/ET)-dependent defense signaling pathways, AtWRKY28 and AtWRKY75 mainly active JA/ET pathway to defend Arabidopsis against S. sclerotiorum and oxalic acid stress.