Cloning and sequencing of a genomic island found in the Brazilian purpuric fever clone of Haemophilus influenzae biogroup aegyptius.
Cloning and sequencing of a genomic island found in the Brazilian purpuric fever clone of Haemophilus influenzae biogroup aegyptius.
复制标题
在埃及流感嗜血杆菌生物群的巴西紫癜克隆中发现的基因组岛的克隆和测序。
DOI：
10.1128/iai.73.4.1927-1938.2005
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Actis,LuisA
中科院分区：
文献类型：
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作者：
McGillivary,Glen;Tomaras,AndrewP;Rhodes,EricR;Actis,LuisA
A genomic island was identified in theHaemophilus influenzaebiogroup aegyptius Brazilian purpuric fever (BPF) strain F3031. This island, which was also found in other BPF isolates, could not be detected in non-BPF biogroup aegyptius strains or in nontypeable or typeableH. influenzaestrains, with the exception of a region present in the type b Eagan strain. This 34,378-bp island is inserted, in reference toH. influenzaeRd KW20, within a choline transport gene and contains a mosaic structure of Mu-like prophage genes, several hypothetical genes, and genes potentially encoding anErwinia carotovoracarotovoricin Er-like bacteriocin. The product of the tail fiber ORF in the bacteriocin-like region shows a hybrid structure where the C terminus is similar to anH. influenzaephage HP1 tail protein implicating this open reading frame in altering host specificity for a putative bacteriocin. Significant synteny is seen in the entire genomic island with genomic regions fromSalmonella entericasubsp.entericaserovar Typhi CT18,Photorhabdus luminescenssubsp.laumondiiTT01,Chromobacterium violaceum, and to a lesser extentHaemophilus ducreyi35000HP. In a previous work, we isolated several BPF-specific DNA fragments through a genome subtraction procedure, and we have found that a majority of these fragments map to this locus. In addition, several subtracted fragments generated from an independent laboratory by using different but related strains also map to this island. These findings underscore the importance of this BPF-specific chromosomal region in explaining some of the genomic differences between highly invasive BPF strains and non-BPF isolates of biogroup aegyptius.
