APPEARANCE OF AN ORNITHINE DECARBOXYLASE INHIBITORY PROTEIN UPON ADDITION OF PUTRESCINE TO CELL-CULTURES
APPEARANCE OF AN ORNITHINE DECARBOXYLASE INHIBITORY PROTEIN UPON ADDITION OF PUTRESCINE TO CELL-CULTURES
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DOI：
10.1016/0304-4165(76)90054-4
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发表时间：
1976-01-01
期刊：
影响因子：
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通讯作者：
CANELLAKIS, ES
中科院分区：
文献类型：
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作者：
FONG, WF;HELLER, JS;CANELLAKIS, ES
静止的，接触抑制的H-35大鼠肝癌细胞培养在最低限度的基本培养基中含有非常低水平的鸟氨酸脱羧酶活性[EC 4.1.1.17]。然而，在培养基中添加10%胎牛血清2 h后，酶活性提高了0.75%。100倍。同时加入10-2 M腐胺可以完全抑制这种增加。腐胺的存在引起细胞内鸟氨酸脱羧酶抑制剂的出现。这种鸟氨酸脱羧酶抑制剂的分子量为26,500，对凝乳胰蛋白酶的作用敏感，对鸟氨酸是非竞争性的。该抑制剂的细胞内外观对环己亚胺敏感，但仅部分被放线菌素D抑制。
Quiescent, contact inhibited H-35 rat hepatoma cell cultures maintained in minimal essential medium contain a very low level of ornithine decarboxylase [EC 4.1.1.17] activity. However, 2 h after the addition of 10% fetal calf serum to the culture medium, the enzyme activity increases by .apprx. 100-fold. This increase can be completely inhibited by the simultaneous addition of 10-2 M putrescine. The presence of putrescine elicits the appearance of an intracellular inhibitor of ornithine decarboxylase. This inhibitor of ornithine decarboxylase has a MW of 26,500, is sensitive to the action of chymotrypsin and is non-competitive with respect to ornithine. The intracellular appearance of this inhibitor is sensitive to cycloheximide but is only partially inhibited by actinomycin D.