Tracer-toxins: cholera toxin B-saporin as a model
Tracer-toxins: cholera toxin B-saporin as a model
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DOI：
10.1016/s0165-0270(00)00298-3
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发表时间：
2000-11-15
影响因子：
3
通讯作者：
Minson, JB
中科院分区：
文献类型：
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作者：
Llewellyn-Smith, IJ;Martin, CL;Minson, JB
我们之前已经证明，在注射后7天，逆行转运的霍乱毒素B （CTB）皂苷可以消除交感神经节前神经元（Llewellyn-Smith, i.j., Martin, c.l., Arnolda, l.f.m Minson, j.b., 1999）。神经病学杂志10,307)。为了确定这种示踪毒素是否能以类似的时间过程杀死其他类型的逆行转运CTB的神经元，我们将CTB皂苷注射到大鼠面神经中，并让它们存活7天。在灌注的髓质切片上对注射神经的同侧和对侧进行面部运动神经元计数，以显示胆碱乙酰转移酶（ChAT）的免疫反应性。在9只大鼠中，有3只大鼠注射神经同侧的chat免疫反应神经元数量有统计学意义的减少。注射量不足可能是大多数大鼠经CTB皂苷处理后运动神经元数量没有减少的原因，因为未偶联CTB逆行示踪后，大鼠之间显示CTB免疫反应性的面部运动神经元的染色强度和数量有明显差异。这些结果表明CTB-皂苷可以消除运动神经元和交感神经节前神经元，表明应该优化注射示踪毒素的方案，以确保最大程度的神经元死亡，并支持我们的观点，即CTB-皂苷应该杀死任何表达GM1神经节苷脂的中枢神经元，GM1神经节苷脂是CTB结合的膜成分。(C) 2000 Elsevier Science B.V.版权所有
We have shown previously that retrogradely-transported cholera toxin B (CTB)-saporin has eliminated sympathetic preganglionic neurons by 7 days after injection (Llewellyn-Smith, I.J., Martin, C.L., Arnolda, L.F., Minson, J.B., 1999. NeuroReport 10, 307). To ascertain whether this tracer-toxin can kill other types of neurons that transport CTB retrogradely with a similar time course, we injected CTB-saporin into the facial nerves of rats and allowed them to survive for 7 days. Facial motoneurons were counted ipsilateral and contralateral to the injected nerves in sections of perfused medulla processed to reveal immunoreactivity for choline acetyltransferase (ChAT). There was a statistically significant decrease in the number of ChAT-immunoreactive neurons ipsilateral to the injected nerve in three out of nine rats. Inadequate injections were probably the reason that most rats showed no decrease in motoneurons numbers after treatment with CTB-saporin, since the staining intensity and numbers of facial motoneurons that showed CTB-immunoreactivity varied markedly between rats after retrograde tracing with unconjugated CTB. These results show that CTB-saporin can eliminate motoneurons as well as sympathetic preganglionic neurons, indicate that protocols for the injection of tracer-toxins should be optimized to ensure maximum neuronal death and support our contention that CTB-saporin should kill any central neuron that expresses GM1 ganglioside, the membrane component to which CTB binds. (C) 2000 Elsevier Science B.V. All rights reserved.