Two transgenic approaches to define the cell lineages in endocrine pancreas development
Two transgenic approaches to define the cell lineages in endocrine pancreas development
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DOI：
10.1016/s0303-7207(98)00028-8
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发表时间：
1998-05-25
影响因子：
4.1
通讯作者：
Vassalli, JD
中科院分区：
文献类型：
--
作者：
Herrera, PL;Orci, L;Vassalli, JD
通过产生转基因小鼠探索胰岛的不同内分泌细胞类型之间的个体发生关系，其中转录胰高血糖素、胰岛素或胰多肽基因的细胞通过白喉毒素A链的启动子靶向表达而被破坏。在另一种方法中，为了评估胰岛素细胞是否来源于产生胰高血糖素或PP的前体，产生了携带胰岛素启动子驱动的和含loxP（“floxed”）报告基因转基因的转基因小鼠，所述报告基因可以通过重组不可逆地“标记”。将它们与在胰高血糖素或PP细胞中表达编码Cre（环化重组）重组酶的另一种转基因（“tagger”）的小鼠杂交。使用这两种方法获得的结果表明，胰高血糖素和胰岛素基因表达细胞都不是其他胰岛细胞的前体;此外，它们表明PP基因表达细胞是胰岛胰岛素和生长抑素细胞分化所必需的，通过细胞谱系或旁分泌关系。(C)1998爱思唯尔科学爱尔兰有限公司保留所有权利。
Ontogenic relationships between the different endocrine cell types of the islets of Langerhans were explored by generating transgenic mice, in which cells transcribing the glucagon, insulin, or pancreatic polypeptide genes were destroyed through the promoter-targeted expression of the diphtheria toxin A chain. In an alternate approach, to assess whether insulin cells are derived from precursors producing glucagon or PP, transgenic mice were generated bearing an insulin promoter-driven, and loxP-containing ('floxed') reporter transgene that can be irreversibly 'tagged' by recombination. They were crossed with mice expressing another transgene ('tagger') encoding Cre (cyclization recombination) recombinase in either glucagon or PP cells. The results obtained using both approaches indicate that neither glucagon nor insulin gene-expressing cells are the precursors to the other islet cells; also, they suggest that PP gene-expressing cells are necessary for the differentiation of islet insulin and somatostatin cells, through a cell lineage or a paracrine relationship. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.