Immune response to cholera toxin epitope inserted in Salmonella flagellin.
Immune response to cholera toxin epitope inserted in Salmonella flagellin.
复制标题
对沙门氏菌鞭毛中插入的霍乱毒素表位的免疫反应。
作者：
S. Newton;C. Jacob;B. Stocker
细菌鞭毛是有效的免疫原，而芳香依赖性 (aro) 沙门氏菌作为活疫苗可引起体液和细胞免疫反应。这种表达保护性抗原表位作为鞭毛蛋白插入物的菌株将提供一种针对由不相关病原体引起的疾病进行疫苗接种的新方法。将指定霍乱毒素 B 亚基表位的合成寡核苷酸插入沙门氏菌鞭毛蛋白基因中。嵌合鞭毛蛋白功能正常，表位在鞭毛表面表达。对表达嵌合鞭毛蛋白基因的都柏林沙门氏菌aro A鞭毛蛋白阴性菌株进行肠胃外给药，可诱发针对霍乱毒素的抗体。
Bacterial flagella are potent immunogens and aromatic-dependent (aro) Salmonella as live vaccines evoke humoral and cellular immune responses. Such strains expressing epitopes of protective antigens as inserts in flagellin would provide a novel way to vaccinate against diseases caused by unrelated pathogens. A synthetic oligonucleotide specifying an epitope of cholera toxin subunit B was inserted in a Salmonella flagellin gene. The chimeric flagellin functioned normally and the epitope was expressed at the flagellar surface. Parenteral administration to mice of an aro A flagellin-negative strain of S. dublin expressing the chimeric flagellin gene evoked antibody to cholera toxin.