Cesium ion desorption ionization with Fourier transform mass spectrometry.
Cesium ion desorption ionization with Fourier transform mass spectrometry.
复制标题
使用傅里叶变换质谱法进行铯离子解吸电离。
DOI：
10.1021/ac00129a020
复制
发表时间：
1987
影响因子：
7.4
通讯作者：
McLafferty,FW
中科院分区：
文献类型：
--
作者：
Amster,IJ;Loo,JA;Furlong,JJ;McLafferty,FW
使用脉冲铯离子枪与傅里叶变换质谱仪连接的二次离子质谱 (SIMS) 对于分析肽和聚合物等非挥发性有机分子具有广泛的用途，所得质谱是实验参数的函数，例如初级离子束的电流密度和加速能量、样品制备技术、样品基质以及共沉积化合物的存在。通过使用高动能 (11 keV) 的低一次离子电流 (20 nA/cm2)，将单层或更少的样品与肽谷胱甘肽共沉积到金靶上，可以获得最佳结果。肽短杆菌肽 S 的检测限为 10" 13 mol。通过这种化合物，串联质谱法可产生额外的结构信息。实验证据支持解吸的中性物质的气相阳离子化的电离机制。
Secondary Ion mass spectrometry (SIMS) using a pulsed cesium Ion gun Interfaced to a Fourier transform mass spectrometer has broad utility for analyzing nonvolatile organic molecules such as peptides and polymers, the resulting mass spectrum Is a function of experimental parameters, such as current density and acceleration energy of the primary Ion beam, sample preparation technique, sample substrate, and presence of codeposited compounds. The best results are obtained for a monolayer or less of a sample codeposited with the peptide glutathione onto a gold target, by using a low primary Ion current (20 nA/cm2) of high kinetic energy (11 keV). A detection limit of 10" 13 mol Is demonstrated for the peptide gramicidin S. With this compound, tandem mass spectrometry Is shown to yield additional structural Information. Experimental evidence supports an Ionization mecha-nism of gas-phase catlonizatlon of the desorbed neutral species.