COMPARISON OF 3 STRAINS OF THE TRANSMISSIBLE GASTROENTERITIS CORONAVIRUS (TGEV) - VIRAL REPLICATION AND SYNTHESIS OF STRUCTURAL ANTIGENS
COMPARISON OF 3 STRAINS OF THE TRANSMISSIBLE GASTROENTERITIS CORONAVIRUS (TGEV) - VIRAL REPLICATION AND SYNTHESIS OF STRUCTURAL ANTIGENS
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DOI：
10.1016/s0769-2617(87)80018-7
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发表时间：
1987-07-01
期刊：
影响因子：
--
通讯作者：
AYNAUD, JM
中科院分区：
文献类型：
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作者：
NGUYEN, TD;BERNARD, S;AYNAUD, JM
将 TGEV 的 Purdue-115 和 D-52 株与通过成年猪胃液中的幸存者选择过程获得的 188-SG 株进行了比较。 188-SG菌株的特征在于(a)低传染性，(b)与RNA合成低和延迟相关的生长延迟和受限，以及(c)结构抗原含量高。相比之下，Purdue-115 和 D-52 毒株的特点是 (a) 高传染性，以及 (b) 病毒复制和 RNA 及结构抗原合成的正常模式。 Tunicamycin 诱导 E1 和 E2 糖蛋白合成的抑制（通过使用单克隆和多克隆抗体的 ELISA 测试检测到）以及 NP 蛋白的显着减少。衣霉素的抑制作用受细胞类型和病毒株的影响。
Purdue-115 and D-52 strains of TGEV were compared with the 188-SG strain, which was obtained by means of a survivor selection process in gastric juice of adult pig. The 188-SG strain was characterized by (a) low infectivity, (b) delayed and restricted growth associated with low and delayed RNA synthesis, and (c) a high content of structural antigens. In contrast, Purdue-115 and D-52 strains were chracterized by (a) high infectivity, and (b) a normal pattern of virus replication and RNA and structural antigen synthesis. Tunicamycin induced the inhibition of synthesis of E1 and E2 glycoproteins (detected by the ELISA test using monoclonal and polyclonal antibodies) as well as a significant reduction in the NP protein. The inhibitory effect of tunicamycin was influenced by the cell type and virus strain.