METHICILLIN-RESISTANT STAPHYLOCOCCI
METHICILLIN-RESISTANT STAPHYLOCOCCI
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DOI：
10.1136/jcp.14.4.385
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发表时间：
1961-01-01
影响因子：
3.4
通讯作者：
BARBER, M
中科院分区：
文献类型：
--
作者：
BARBER, M
18株金黄色葡萄球菌。化脓菌(9株青霉素敏感株和9株破坏青霉素株)在Celbenin平板上传代40~50次。所有菌株对青霉素类抗生素的抗药性都有所提高，其中8株(4株青霉素敏感菌株和4株破坏青霉素菌株)能在100[亩]g/ml或更多的青霉素中生长。耐药类型为耐药型，所有培养物均未灭活Celbenin。对青霉素的耐受性也相应增加。青霉素耐药葡萄球菌与青霉素敏感株和体外分离的青霉素G耐药葡萄球菌的培养特性、凝血酶活性、溶血活性和小鼠毒力基本一致，且在无青霉素存在的情况下反复传代后，耐药程度保持稳定。三种自然产生的耐Celbenin的葡萄球菌菌株。对从感染过程中分离到的化脓性细菌也进行了研究。这三个菌株在塞尔宾浓度高达12-5[亩]克/毫升时都生长旺盛，但在较高浓度时生长很差。对这些发现的可能意义进行了讨论。
Eighteen strains of Staph. pyogenes (nine penicillin-sensitive and nine penicillin-destroying) were passaged 40 to 50 times on Celbenin ditch plates. All strains developed an increase in resistance to Celbenin and eight strains (4 penicillin-sensitive and four penicillin-destroying) were able to grow in 100 [mu]g/ml/or more Celbenin. Resistance was of the drug-tolerant type and none of the cultures inactivated Celbenin. There was an associated increase in tolerance to benzyl penicillin. The highly Celbenin-resistant cultures isolated from penicillin-destroying staphylococci were in sharp contrast to those from penicillin-sensitive strains, as well as to penicillin G-tolerant staphylococci isolated in vitro, because they retained the cultural characteristics, coagulase and hemolytic activity, and mouse virulence of the parent strains, and the degree of resistance remained stable after repeated passage in the absence of Celbenin. Three naturally occurring Celbenin-resistant strains of Staph. pyogenes isolated from infective processes were also studied. All three strains grew luxuriantly in concentrations of Celbenin up to 12-5 [mu]g/ml. but very poorly in higher concentrations. The possible significance of these findings is discussed.