The suitability of laboratory-bred Anopheles cracens for the production of Plasmodium vivax sporozoites.
The suitability of laboratory-bred Anopheles cracens for the production of Plasmodium vivax sporozoites.
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DOI：
10.1186/s12936-015-0830-0
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发表时间：
2015-08-12
期刊：
影响因子：
3
通讯作者：
Nosten F
中科院分区：
文献类型：
--
作者：
Andolina C;Landier J;Carrara V;Chu CS;Franetich JF;Roth A;Rénia L;Roucher C;White NJ;Snounou G;Nosten F
在20年前，在泰国绝缘层建立的稳定的菌落（A. dirus b）被证明对疟原虫易感。 A. cracens菌落在泰国山边界的绝缘层中，以及使用维瓦克斯田间样品生产传染性孢子菌的过程。
该殖民地始于2012年，该鸡蛋批次是从Chiang Mai大学的寄生虫学系寄到了Shoklo Malaria Research Mution（SMRU）的，该单元（SMRU）从2013年5月的湿滤纸上送到了湿滤纸。 2014年12月，在SMRU诊所寻求护理的患者中收集的Vivax P.感染的血液样本用于膜进食测定法和孢子虫群落的产生。
蚊子以55例患者的血液样本为食，这导致了生产的孢子虫，每个蚊子获得的孢子虫的平均数量为26,112（范围为328-79,310）。 = 0.82），或产生的孢子虫数量（Spearman的ρ= -0.016，p = 0.905）。 ρ= 0.179，p = 0.282）。
一致的和常规的孢子孢子的生产证实，A. cracens非常容易受到该载体的实验室繁殖菌落的影响。
本文的在线版本（doi：10.1186/s12936-015-0830-0）包含补充材料，可供授权用户使用。
A stenogamous colony of Anopheles cracens (A. dirus B) established 20 years ago in a Thai insectary proved susceptible to Plasmodium vivax. However, routine sporozoite production by feeding on field-collected blood samples has not been described. The setting-up of an A. cracens colony in an insectary on the Thai-Myanmar border and the process of using P. vivax field samples for the production of infectious sporozoites are described. The colony was started in 2012 from egg batches that were sent from the Department of Parasitology, Faculty of Medicine, University of Chiang Mai, to the Shoklo Malaria Research Unit (SMRU), on wet filter paper in sealed Petri dishes. From May 2013 to December 2014, P. vivax-infected blood samples collected from patients seeking care at SMRU clinics were used for membrane feeding assays and sporozoite production. Mosquitoes were fed on blood samples from 55 patients, and for 38 (69 %) this led to the production sporozoites. The average number of sporozoites obtained per mosquito was 26,112 (range 328–79,310). Gametocytaemia was not correlated with mosquito infectiousness (p = 0.82), or with the number of the sporozoites produced (Spearman’s ρ = −0.016, p = 0.905). Infectiousness did not vary with the date of collection or the age of the patient. Mosquito survival was not correlated with sporozoite load (Spearman’s ρ = 0.179, p = 0.282). Consistent and routine P. vivax sporozoites production confirms that A. cracens is highly susceptible to P. vivax infection. Laboratory-bred colonies of this vector are suitable for experimental transmission protocols and thus constitute a valuable resource. The online version of this article (doi:10.1186/s12936-015-0830-0) contains supplementary material, which is available to authorized users.