Protocadherins control the modular assembly of neuronal columns in the zebrafish optic tectum.
Protocadherins control the modular assembly of neuronal columns in the zebrafish optic tectum.
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DOI：
10.1083/jcb.201507108
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发表时间：
2015-11-23
期刊：
影响因子：
--
通讯作者：
Jontes JD
中科院分区：
文献类型：
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作者：
Cooper SR;Emond MR;Duy PQ;Liebau BG;Wolman MA;Jontes JD
δ-原钙粘蛋白将斑马鱼视顶盖分隔成径向神经元柱，并且柱内的神经元是来自共同神经元祖细胞的兄弟姐妹。细胞间识别指导脊椎动物大脑在发育过程中的组装。 δ-原钙粘蛋白包含一个神经粘附分子家族，这些分子具有差异表达并与一系列神经发育障碍有关。在这里，我们表明δ-原钙粘蛋白的表达将斑马鱼视顶盖划分为放射状神经元柱。利用细菌人工染色体转基因斑马鱼的体内双光子成像，我们发现 pcdh19 在离散的神经元柱中表达，并且这些柱状模块源自增殖的 pcdh19+ 神经上皮前体。消除 pcdh19 会导致柱状组织破坏和视觉引导行为缺陷。这些结果揭示了组织发育中的神经系统的基本机制：将早期神经上皮细分为具有不同分子特性的前体，引导平行神经元单元的自主发育，组织神经回路的形成和行为。
δ-Protocadherins partition the zebrafish optic tectum into radial columns of neurons, and the neurons within a column are siblings derived from common neuronal progenitors. Cell–cell recognition guides the assembly of the vertebrate brain during development. δ-Protocadherins comprise a family of neural adhesion molecules that are differentially expressed and have been implicated in a range of neurodevelopmental disorders. Here we show that the expression of δ-protocadherins partitions the zebrafish optic tectum into radial columns of neurons. Using in vivo two-photon imaging of bacterial artificial chromosome transgenic zebrafish, we show that pcdh19 is expressed in discrete columns of neurons, and that these columnar modules are derived from proliferative pcdh19+ neuroepithelial precursors. Elimination of pcdh19 results in both a disruption of columnar organization and defects in visually guided behaviors. These results reveal a fundamental mechanism for organizing the developing nervous system: subdivision of the early neuroepithelium into precursors with distinct molecular identities guides the autonomous development of parallel neuronal units, organizing neural circuit formation and behavior.