Circadian clocks go in vitro: purely post-translational oscillators in cyanobacteria.
Circadian clocks go in vitro: purely post-translational oscillators in cyanobacteria.
复制标题
生物钟在体外：蓝藻中的纯粹翻译后振荡器。
DOI：
10.1038/msb4100027
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发表时间：
2005
影响因子：
9.9
通讯作者：
Naef,Felix
中科院分区：
文献类型：
--
作者：
Naef,Felix
最近关于蓝藻中昼夜节律振荡器核心的发现正在挑战这种时钟是通过转录-翻译反馈调节驱动的教条。相反，主起搏器独立于转录和翻译，由体内KaiC蛋白磷酸化状态的自我持续振荡组成。使用三种重组蛋白与三磷酸腺苷的最小混合物，甚至在体外复制了核心时钟。由此产生的化学振荡器可以精确地再现突变体的温度补偿和变周期表型。这个系统现在提供了一个理想的游乐场，通过添加连续的组件来重建生物钟，同时用化学分辨率理解每一个步骤。
Recent findings about the core of the circadian oscillator in cyanobacteria are challenging the dogma that such clocks are driven through transcriptional–translational feedback regulation. Instead, the master pacemaker is independent of both transcription and translation, and consists of self‐sustained oscillations in the phosphorylation status of the KaiC proteinin vivo. Using a minimal cocktail of three recombinant proteins with adenosine triphosphate, the core clock was even reproducedin vitro. The so‐born chemical oscillator could reproduce accurately temperature compensation and altered period phenotypes in mutants. This system now provides an ideal playground for rebuilding the circadian clock by adding successive components while understanding every single step with chemical resolution.