Diversity of fungal isolates from three Hawaiian marine sponges
Diversity of fungal isolates from three Hawaiian marine sponges
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DOI：
10.1016/j.micres.2007.07.002
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发表时间：
2009-01-01
影响因子：
6.7
通讯作者：
Wang, Guangyi
中科院分区：
文献类型：
--
作者：
Li, Quanzi;Wang, Guangyi
海绵是多种原核和真核微生物的栖息地。然而，海绵真菌结合的性质和海绵衍生真菌的多样性几乎没有得到解决。在本研究中，采用培养依赖方法研究了夏威夷海绵纤维胶海绵、海绵海绵和阿玛塔海绵的真菌多样性。所培养的真菌分别属于子囊菌门25属和担子菌门1属8个目。这些分离株中有一部分（n = 15,17 %）与从其他海洋生境分离的真菌有密切的亲缘关系；其余分离株与陆生真菌菌株有亲缘关系。培养的真菌分离物被分为3组：“海绵通用型”（在所有海绵物种中发现）、“海绵联系型”（在不止一种海绵物种中发现）和“海绵专门型”（仅在一种海绵物种中发现）。在两个不同地点收集的纤维丝状纤维瘤个体共享同一组“海绵专家”。在海绵中首次鉴定出15个代表性属。大规模的海绵衍生真菌系统发育分析可能提供关键的。在海绵中区分“常驻真菌”和“瞬时真菌”的信息，就像在其他海洋微生物群中所做的那样。本文首次报道了海绵中可培养真菌群落的宿主特异性分析。(C) 2007 Elsevier GmbH版权所有。
Sponges harbor diverse prokaryotic and eukaryotic microbes. However, the nature of sponge-fungal association and diversity of sponge-derived fungi have barely been addressed. In this study, the cultivation-dependent approach was applied to study fungal diversity in the Hawaiian sponges Gelliodes fibrosa, Haliclona caerulea, and Mycale armata. The cultivated fungal isolates were representatives of 8 taxonomic orders, belonging to at least 25 genera of Ascomycota and 1 of Basidiomycota. A portion of these isolates (n = 15, 17%) were closely affiliated with fungal isolates isolated from other marine habitats; the rest of the isolates had affiliation with terrestrial fungal strains. Cultivated fungal isolates were classified into 3 groups: 'sponge-generalists'-found in all sponge species, 'sponge-associates'-found in more than one sponge species, and 'sponge-specialists'-found only in one sponge species. Individuals of G. fibrosa collected at two different locations shared the same group of 'sponge-specialists'. Also, representatives of 15 genera were identified for the first time in marine sponges. Large-scale phylogenetic analysis of sponge-derived fungi may provide critical. information to distinguish between,resident fungi' and 'transient fungi' in sponges as it has been done in other marine microbial groups. This is the first report of the host specificity analysis of culturable fungal communities in marine sponges. (C) 2007 Elsevier GmbH. All rights reserved.