Relative Contributions of 5-Hydroxytryptamine (5-HT) Receptor Subtypes in 5-HT-Induced Vasoconstriction of the Distended Human Saphenous Vein as a Coronary Artery Bypass Graft
Relative Contributions of 5-Hydroxytryptamine (5-HT) Receptor Subtypes in 5-HT-Induced Vasoconstriction of the Distended Human Saphenous Vein as a Coronary Artery Bypass Graft
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DOI：
10.1248/bpb.34.82
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发表时间：
2011-01-01
影响因子：
2
通讯作者：
Yamamoto, Ryuichi
中科院分区：
文献类型：
--
作者：
Nakamura, Eisaku;Tanaka, Naoko;Yamamoto, Ryuichi
已经确定，在冠状动脉旁路移植术（CABG）手术中广泛用作管道血管的隐静脉（SV）段在植入冠状动脉循环之前用高压扩张以检查泄漏并增加通畅性。本研究的目的是阐明5-羟色胺（5-HT）受体亚型负责5-HT诱导的血管收缩扩张的人SV的相对贡献。而大约一半的5-HT-诱导的血管收缩仍然存在于超最大浓度的沙格雷酯或SB 224289（5-HT 2A和5-HT 1B受体拮抗剂，分别），同时治疗沙格雷酯和SB 224289几乎完全抑制5-HT-诱导的血管收缩。5-HT_（2A）和5-HT_（1B）受体在扩张的人SV平滑肌细胞中呈免疫阳性染色，5-HT_（2A）和5-HT_（1B）受体的免疫阳性面积无显著性差异。这些结果表明，5-HT 2A和5-HT 1B受体同样有助于5-HT诱导的人扩张SV的血管收缩。因此，当SV用作CABG导管时，5-HT 2A和5-HT 1B受体拮抗剂的组合似乎对预防5-HT诱导的痉挛最有用。
It is established that the segment of saphenous vein (SV) that is widely used as a conduit vessel in coronary artery bypass graft (CABG) surgery is distended with high pressure to check for leaks and to increase the patency before implantation into coronary arterial circulation. The aim of the present study was to elucidate the relative contributions of 5-hydroxytryptamine (5-HT) receptor subtypes responsible for 5-HT-induced vasoconstriction of the distended human SV. Whereas about half of the 5-HT-induced vasoconstriction still remained in the presence of supramaximum concentration of sarpogrelate or of SB224289 (5-HT2A and 5-HT1B receptor antagonists, respectively), simultaneous treatment with sarpogrelate and SB224289 almost completely inhibited the 5-HT-induced vasoconstriction. Immunopositive staining for 5-HT2A and 5-HT1B receptors was detected in smooth muscle cells of the distended human SV and there was no significant difference between the immunopositive areas of 5-HT2A and 5-HT1B receptors. These results demonstrate that 5-HT2A and 5-HT1B receptors similarly contribute to 5-HT-induced vasoconstriction in human distended SV. Thus, when the SV is used as a CABG conduit, a combination of 5-HT2A and 5-HT1B receptor antagonists would appear to be most useful to prevent 5-HT-induced spasm.