Top-Down Mass Spectrometry: Proteomics to Proteoforms.
Top-Down Mass Spectrometry: Proteomics to Proteoforms.
复制标题
自上而下的质谱分析：蛋白质组学到蛋白质形式。
DOI：
10.1007/978-3-319-41448-5_8
复制
发表时间：
2016
影响因子：
--
通讯作者：
Patrie,StevenM
中科院分区：
文献类型：
--
作者：
Patrie,StevenM
本章重点介绍了自上而下质谱 (TDMS) 领域的许多基本概念和技术，并提供了 TD 在生物学、生物物理学和临床研究方面做出的贡献的大量示例。 TD 工作流程包括多种步骤，其中可能包括非特异性或靶向制备策略、正交液相色谱技术、分析物电离、质量分析、串联质谱 (MS/MS) 和信息学程序。这种多样性的实验设计已经发展到可以管理蛋白质组研究中蛋白质表达的大动态范围和蛋白质的不同理化性质，解决蛋白质形式的微观异质性，以及确定气相蛋白质和蛋白质组装体的结构和组成。
This chapter highlights many of the fundamental concepts and technologies in the field of top-down mass spectrometry (TDMS), and provides numerous examples of contributions that TD is making in biology, biophysics, and clinical investigations. TD workflows include variegated steps that may include non-specific or targeted preparative strategies, orthogonal liquid chromatography techniques, analyte ionization, mass analysis, tandem mass spectrometry (MS/MS) and informatics procedures. This diversity of experimental designs has evolved to manage the large dynamic range of protein expression and diverse physiochemical properties of proteins in proteome investigations, tackle proteoform microheterogeneity, as well as determine structure and composition of gas-phase proteins and protein assemblies.