Tissue-specific and time-coordinated hormone regulation of plasminogen-activator-inhibitor type I and tissue-type plasminogen activator in the rat ovary during gonadotropin-induced ovulation.
Tissue-specific and time-coordinated hormone regulation of plasminogen-activator-inhibitor type I and tissue-type plasminogen activator in the rat ovary during gonadotropin-induced ovulation.
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DOI：
10.1111/j.1432-1033.1991.tb15736.x
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Y. Liu;X. Peng;T. Ny
中科院分区：
文献类型：
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作者：
Y. Liu;X. Peng;T. Ny
纤溶酶原激活剂系统在许多生物过程中提供蛋白水解活性。纤溶酶原激活的调节可能发生在许多水平，包括纤溶酶原激活物（PA）的合成和分泌以及抑制剂对PA活性的特异性抑制。PA抑制剂1型（派-1）是组织型PA（tPA）和尿激酶型PA（uPA）的有效抑制剂，因此可能有助于控制纤溶酶原激活。为了研究PA和PA抑制剂的协调调节是否在体内响应生理信号而发生，我们研究了促性腺激素诱导排卵期间卵巢中派-1和tPA的调节。我们发现，派-1，以及tPA的活性和mRNA水平，协调调节促性腺激素在一个时间依赖性和细胞特异性的方式，这样的PA活性激增，获得排卵前。卵泡膜间质细胞和颗粒细胞合成派-1，但其最大派-1的表达发生在不同的时间在排卵期，确保抑制蛋白水解活性在卵巢细胞外室之前和之后排卵。卵巢中tPA和派-1的协调调节可能微调PA活性的峰值，这可能对排卵过程的调节是重要的。
The plasminogen-activator system provides proteolytic activity in many biological processes. The regulation of plasminogen activation may occur at many levels including the synthesis and secretion of plasminogen activators (PA) and the specific inhibition of PA activity by inhibitors. PA-inhibitor type-1 (PAI-1) is an efficient inhibitor of tissue-type PA (tPA) and urokinase-type PA (uPA) that may therefore be instrumental for the control of plasminogen activation. To investigate if coordinated regulation of PA and PA inhibitors take place in vivo in response to physiological signals, we have examined the regulation of PAI-1 and tPA in the ovary during gonadotropin-induced ovulation. We found that PAI-1, as well as tPA activity and mRNA levels, were coordinately regulated by gonadotropins in a time-dependent and cell-specific manner, such that a surge of PA-activity was obtained just prior to ovulation. Both theca-interstitial and granulosa cells synthesized PAI-1, but their maximal PAI-1 expression occurred at different times during the periovulatory period, ensuring inhibition of proteolytic activity in ovarian extra cellular compartments both before and after ovulation. The coordinated regulation of tPA and PAI-1 in the ovary may fine-tune the peak of PA activity which may be important for the regulation of the ovulatory process.