IRON-CONTAINING AND MANGANESE-CONTAINING SUPEROXIDE DISMUTASES CAN BE DISTINGUISHED BY ANALYSIS OF THEIR PRIMARY STRUCTURES
IRON-CONTAINING AND MANGANESE-CONTAINING SUPEROXIDE DISMUTASES CAN BE DISTINGUISHED BY ANALYSIS OF THEIR PRIMARY STRUCTURES
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DOI：
10.1016/0014-5793(88)81160-8
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发表时间：
1988-03-14
期刊：
影响因子：
3.5
通讯作者：
BLAKE, CCF
中科院分区：
文献类型：
--
作者：
PARKER, MW;BLAKE, CCF
含铁和含锰的超氧化物歧化酶具有非常相似的三维结构，但可以通过各种生化手段加以区分。已知六种含锰和三种含铁的超氧化物歧化酶的初级结构。对这些酶的排列氨基酸序列的分析以及X射线衍射研究的结构数据表明，这两类酶可以根据位于酶活性位点或靠近活性位点的少量单位点取代来区分。
The iron‐ and manganese‐containing superoxide dismutases have very similar three‐dimensional structures but can be distinguished by various biochemical means. The primary structures of six manganese‐containing and three iron‐containing superoxide dismutases are known. Analysis of the aligned amino acid sequences of these enzymes taken together with structural data from X‐ray diffraction studies demonstrates that the two classes of enzyme can be distinguished on the basis of a small number of single‐site substitutions that are positioned in and close to the active site of the enzyme.