Luminol-based ternary electrochemiluminescence nanospheres as signal tags and target-triggered strand displacement reaction as signal amplification for highly sensitive detection of Helicobacter pylori DNA
Luminol-based ternary electrochemiluminescence nanospheres as signal tags and target-triggered strand displacement reaction as signal amplification for highly sensitive detection of Helicobacter pylori DNA
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基于鲁米诺的三元电化学发光纳米球作为信号标签和靶标触发的链置换反应作为信号放大，用于幽门螺杆菌 DNA 的高灵敏检测
DOI：
10.1016/j.snb.2019.05.013
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Zhang Juan
中科院分区：
文献类型：
--
作者：
Tang Hejun;Chen Weixian;Li D;an;Duan Xiaolei;Ding Shijia;Zhao Min;Zhang Juan
本文首次制备了一种新型三元电化学发光（ECL）纳米球，其发射体为发光胺（Lum）、助反应剂为聚乙烯亚胺（PEI）、助反应促进剂为氨基端苝衍生物（PTC-NH2），由于自增强PEI-Lum的分子内助反应和PTC-NH2与Lum/ h2o2体系的分子间助反应加速，该纳米球具有较强的ECL发光能力。将基于lumm的三元ECL纳米球与靶触发链位移反应相结合，构建了一种高灵敏度、无酶的幽门螺杆菌dna生物传感器。所建立的ECL生物传感方法对幽门螺杆菌的检测具有10 fM ~ 10 nM的宽线性范围，检测限低至2.4 fM。因此，本研究为开发高性能ECL纳米材料和无酶生物检测技术应用于临床胃病分子诊断提供了新的途径。
Herein, a novel ternary electrochemiluminescence (ECL) nanosphere containing the emitter of luminol (Lum), the coreactant of polyethylenimine (PEI) and the coreaction accelerator of amino-terminated perylene derivative (PTC-NH2) was prepared for the first time, which possessed strong ECL emission due to intramolecular coreaction of self-enhanced PEI-Lum and intermolecular coreaction acceleration of PTC-NH2to Lum/H2O2system. Combining the Lum-based ternary ECL nanosphere with target-triggered strand displacement reaction, a highly sensitive and enzyme-free biosensor was constructed for determination ofHelicobacter pyloriDNA. This developed ECL biosensing method exhibited a wide linear range from 10 fM to 10 nM and a detection limit down to 2.4 fM forHelicobacter pyloriDNA detection. Therefore, this work provides a new avenue to develop high-performance ECL nanomaterials and an enzyme-free bioassay to be applied in the clinical molecular diagnosis of gastric diseases.