Circulating IGF-binding protein 7 (IGFBP7) levels are elevated in patients with endometriosis or undergoing diabetic hemodialysis
Circulating IGF-binding protein 7 (IGFBP7) levels are elevated in patients with endometriosis or undergoing diabetic hemodialysis
复制标题
DOI：
10.1186/1477-7827-6-54
复制
发表时间：
2008-11-19
影响因子：
4.4
通讯作者：
Tamura, Kazuhiro
中科院分区：
文献类型：
--
作者：
Kutsukake, Masahiko;Ishihara, Ryosuke;Tamura, Kazuhiro
背景：胰岛素样生长因子结合蛋白7（IGFBP7）是一种分泌性蛋白，分子量约为30 kDa。它在月经周期的分泌期大量表达于子宫内膜。IGFBP7的表达减少已被观察到在一些癌症和leiomata.Methods：为了确定是否血清IGFBP7水平反映子宫IGFBP7表达在人类月经周期的变化，并检查是否血清IGFBP7水平改变患者的各种疾病，我们开发了一种新的，双抗体夹心酶联免疫吸附试验（ELISA）。首先，在培养的子宫内膜基质细胞和腺细胞中测定释放到培养基中的IGFBP7的浓度。血液样本采集自月经周期正常和被诊断患有子宫内膜异位症的妇女。血液透析患者和胃肠道肿瘤患者的血清也被用于测定IGFBP7的水平。结果：使用这种新的ELISA，我们证明培养的子宫细胞分泌IGFBP7到培养基中。子宫内膜异位症患者和接受血液透析的II型糖尿病患者的血清IGFBP7浓度显著高于相关对照组。在月经周期的不同阶段，女性的血清IGFBP7水平没有差异。此外，血清IGFBP7水平与结直肠癌，食管癌，或子宫内膜癌患者与正常健康subjects.Conclusion：我们的观察表明，IGFBP7与子宫内膜异位症和糖尿病的病理生理学，血清IGFBP7水平并不反映增强子宫表达IGFBP7 mRNA在月经周期。
Background: Insulin-like growth factor-binding protein-7 (IGFBP7) is a secretory protein with a molecular mass of approximately 30 kDa. It is abundantly expressed in the uterine endometrium during the secretory phase of the menstrual cycle. Decreased IGFBP7 expression has been observed in some cancers and leiomyomata.Methods: To determine whether serum IGFBP7 levels reflect changes in uterine IGFBP7 expression in humans during the menstrual cycle, and to examine whether serum IGFBP7 levels are altered in patients with various disorders, we developed a novel, dual-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Firstly, concentrations of IGFBP7 released into the medium were determined in cultured endometrial stromal and glandular cells. Blood samples were collected from women who had normal menstrual cycles and who had been diagnosed with endometriosis. Serum from hemodialysis patients and gastrointestinal cancers was also used to determine the IGFBP7 levels.Results: Using this new ELISA, we demonstrated that cultured uterine cells secrete IGFBP7 into the medium. Patients with endometriosis and those with type II diabetes mellitus undergoing hemodialysis had significantly higher serum concentrations of IGFBP7 than the relevant control subjects. There were no differences in serum IGFBP7 levels in women at different stages of the menstrual cycle. Furthermore, serum IGFBP7 levels in patients with colorectal, esophageal, or endometrial cancer were not different than normal healthy subjects.Conclusion: Our observations suggest that IGFBP7 is associated with the pathophysiology of endometriosis and diabetes mellitus, and that serum IGFBP7 levels do not reflect enhanced uterine expression of IGFBP7 mRNA during the menstrual cycle.