Intestinal basolateral membrane Ca-ATPase activity with properties distinct from those of the Ca-pump.
Intestinal basolateral membrane Ca-ATPase activity with properties distinct from those of the Ca-pump.
复制标题
肠基底外侧膜 Ca-ATP 酶活性与 Ca 泵的特性不同。
DOI：
10.1016/s0006-291x(86)80140-1
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发表时间：
1986
影响因子：
3.1
通讯作者：
Weiser,MM
中科院分区：
文献类型：
--
作者：
Moy,TC;Walters,JR;Weiser,MM
以往的研究表明，大鼠肠道基底膜上的钙泵是囊泡型ATP依赖的钙摄取。在目前的研究中，测量了钙刺激的ATP水解酶活性(Ca-ATPase活性)，发现它与钙泵的不同之处在于它具有更高的活性，并且对钒酸不敏感。尽管该泵是专用于ATP的，但对ATP、GTP或ADP有水解性，但对AMP或对硝基苯磷酸没有水解性。与钙泵活性相反，不同肠段、十二指肠绒毛/隐窝细胞组和维生素D缺乏动物的Ca-ATPase活性相似。因此，正如通常测量的那样，肠基底侧膜Ca-ATPase活性并不等同于钙泵。
The Ca-pump in rat intestinal basolateral membranes had been studied previously as vesicular ATP-dependent Ca-uptake. In the present studies, Ca-stimulated ATP hydrolysis (Ca-ATPase activity) was measured and found to differ from the Ca-pump in having higher activity and being insensitive to vanadate. Whereas the pump was specific for ATP, hydrolytic activity was found with ATP, GTP or ADP but not with AMP or p-nitro-phenyl-phosphate. In contrast to Ca-pump activity, Ca-ATPase activities were similar for different intestinal segments, for duodenal villus/crypt cell-fractions and for vitamin D-deficient animals. Thus, as usually measured, intestinal basolateral membrane Ca-ATPase activity is not equivalent to the Ca-pump.